1.24823

Nitro blue tetrazolium chloride (NBT)

for microscopy

• Synonym(s): Nitro blue tetrazolium chloride (NBT), 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis [2-(4-nitrophenyl)-5-phe nyl-2H-tetrazolium chloride], NBT
• CAS Number: 298-83-9
• Molecular Weight: 817.64
• MDL number: MFCD00012159
• UNSPSC Code: 41116124
• EC Index Number: 206-067-4

Properties

• Quality Level: 400
• form: solid
• mp: 189 °C
• εmax: 800-900 at 256-259 nm in methanol at 0.01 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• InChI: 1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
• InChI key: FSVCQIDHPKZJSO-UHFFFAOYSA-L

Description

General description

Nitro blue tetrazolium chloride (NBT) is a dry dye for the staining of histological tissue sections and histochemical as well as in vivo methods on material of human origin.
The dye is a CE certified IVD product and has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

application

Dry dye used in histochemistry

Analysis Note

Identity (UV/VIS-Spectrum)passes testAbsorption maximum λ max (Methanol)256 - 259 nmSpec. Absorptivity A 1%/1cm (λ_max; 0.01 g/l; methanol; calc. on dried substance)800 - 900Loss on drying (Vacuum < 7 mbar, P₂O₅, 24 h)≤ 12 %Suitability for microscopypasses test

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H302 - H315 - H319 - H335
• pcodes: P261 - P264 - P270 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_c: Not applicable