1.08178

Trifluoroacetic acid

for protein sequence analysis

• Synonym(s): TFA
• Linear Formula: CF₃COOH
• CAS Number: 76-05-1
• Molecular Weight: 114.02
• Beilstein/REAXYS Number: 742035
• MDL number: MFCD00004169
• UNSPSC Code: 12352106
• EC Index Number: 200-929-3
• NACRES: NA.21

Properties

• vapor density: 3.9 (vs air)
• Quality Level: 200
• vapor pressure: 97.5 mmHg ( 20 °C)
• assay: ≥99.7% (acidimetric)
• form: liquid
• impurities: ≤0.02% Water (Karl Fischer)
• refractive index: n20/D 1.3 (lit.)
• pH: 1 (10 g/L in H₂O)
• bp: 72.4 °C (lit.)
• mp: −15.4 °C (lit.)
• solubility: soluble 10 g/mL
• density: 1.489 g/mL at 20 °C (lit.)
• storage temp.: 2-30°C
• SMILES string: OC(C(F)(F)F)=O
• InChI: 1S/C2HF3O2/c3-2(4,5)1(6)7/h(H,6,7)
• InChI key: DTQVDTLACAAQTR-UHFFFAOYSA-N

Description

Application

Trifluoroacetic acid (TFA) can be used as an ion-pairing agent in a reverse-phase HPLC system to separate peptides because it enhances peak resolution. It also has low absorption detection wavelengths and is relatively volatile and can be easily removed. For the majority of peptide separations, TFA has been used at concentrations ranging from 0.05 to 0.1%(6.5-13 mM).

Analysis Note

Assay (acidimetric): ≥ 99.7 %
Identity (IR-spectrum): passes test
Density (d 20 °C/20 °C): about 1.53
Boiling point: about 72 °C
Water (according to Karl Fischer): ≤ 0.02 %

Safety Information

• pictograms: GHS05,GHS07
• signalword: Danger
• hcodes: H314 - H332 - H412
• pcodes: P261 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1A
• Storage Class: 8A - Combustible, corrosive hazardous materials
• wgk_germany: WGK 2
• flash_point_f: 212.0 °F - Pensky-Martens closed cup
• flash_point_c: > 100 °C - Pensky-Martens closed cup