07-449

Anti-trimethyl-Histone H3 (Lys27) Antibody

Upstate^®, from rabbit

• Synonym(s): H3 histone family, member T, histone 3, H3, histone cluster 3, H3, H3k27me3
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: human, mouse
• Application: ICC, IHC, IP, WB, multiplexing
• Citations: 2256

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, mouse
• manufacturer/tradename: Upstate^®
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, multiplexing: suitable, western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_003493

Description

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

17 kDa

Immunogen

KLH-conjugated, synthetic 2X-branched peptide containing the sequence …AR(me3K)SAP… in which me3K corresponds to trimethyl-lysine at residue 27 of human histone H3.

Application

Anti-trimethyl-Histone H3 (Lys27), also known as Anti-H3K27me3, is a highly published Rabbit Polyclonal Antibody. This protein A purified antibody is dot blot tested for trimethylated lysine 27 specificity and validated in WB, ICC, IP.

Immunocytochemistry:

Immunoprecipitation:

Beadlyte Histone-Peptide Specificity Assay:

Biochem/physiol Actions

Trimethylated histone H3 (Lys27). Broad species cross-reactivity expected.

Broad species cross-reactivity expected.

Physical form

Format: Purified

Purified rabbit IgG in buffer containing 0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide before the addition of 30% glycerol.

Analysis Note

Routinely evaluated by western blot in acid extracted proteins from HeLa cells.

Western Blot Analysis:
0.4 µg/mL of anti-trimethyl histone H3 Lys27 was used on recombinant Histone H3 and HeLa cell acid extracts.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1