07-2270

Anti-WNK4 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Serine/threonine-protein kinase WNK4, Protein kinase lysine-deficient 4, Protein kinase with no lysine 4
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: rabbit
• Quality Level: 100
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, mouse
• species reactivity (predicted by homology): rat (based on 100% sequence homology)
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI accession no.: NP_115763
• UniProt accession no.: Q96J92
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... WNK4(65266)

Description

General description

WNK4 is a serine-threonine protein kinase that is commonly expressed in the distal nephron. Mutations in WNK1 and WNK4 cause pseudohypoaldosteronism type II, which causes hypertension, hyperkalemia, hyperchloremia and metabolic acidosis. WNK4 has been shown to decrease Na-Cl cotransporter activity and the potassium channel by removing it from the cell surface. It is thought that activating WNK4 inhibits the activity of WNK1. WNK4 has been shown to play a role in regulating blood pressure in addition to potassium homeostasis.

Specificity

This antibody recognizes WNK4 at the C-terminus.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to human WNK4 at the C-terminus.

Application

Immunohistochemistry Analysis: 1:1,000 dilution from a previous lot detected WNK4 in normal human kidney tissue.

Research Category
Signaling

Research Sub Category
Kinases & Phosphatases

This WNK4 antibody is validated for use in WB & IHC for the detection of the WNK4 protein.

Quality

Evaluated by Western Blot in mouse kidney cell lysate.

Western Blot Analysis: 0.05 µg/mL of this antibody detected WNK4 on 10 µg of mouse kidney cell lysate.

Target description

~160 kDa observed. This protein has 3 isoforms produced by alternative splicing. Hengl, T., et al. (2010). PNAS. 107(13):6052–6057.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse kidney cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable