07-1614

Anti-XRCC2 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): DNA repair protein XRCC2, X-ray repair complementing defective repair in Chinese hamster cells 2, X-ray repair cross complementing protein 2, X-ray repair cross-complementing protein 2, X-ray repair, complementing defective, repair in Chinese hamster
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, mouse
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI accession no.: NM_005431
• UniProt accession no.: O43543
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... XRCC2(7516)

Description

General description

X-ray repair cross-complementing protein (XRCC2) is a member of the RecA family which is essential for DNA repair and maintenance. XRCC2 also belongs to the Rad51 subfamily of proteins involved in double stranded DNA break repair. XRCC2 is thought to encode a member of the RecA/Rad51 related protein family which maintains chromosome stability and repairs DNA damage. XRCC2 and RAD51 interact to form a multimeric ring in the absence of DNA. XRCC2 is thought to load Rad51 onto gapped DNA.

Specificity

This antibody recognizes XRCC2.

Immunogen

A synthetic peptide corresponding to amino acids 206-217 of XRCC2.

application

Immunocytochemistry:
Anti-XRCC2 polyclonal was used in immunocytochemistry to positively stain the nucleus on A431, NIH/3T3 and HeLa cells.

Immunohistochemistry(paraffin): Anti-XRCC2 antibody was used for immunohistochemistry on human lung tissue.
Formalin-fixed and paraffin-embedded Human lung tissue stained with anti-XRCC2 (1:100) with Citrate pH 6.0 (HIER).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Use Anti-XRCC2 Antibody (rabbit polyclonal antibody) validated in IHC(P), WB, ICC to detect XRCC2 also known as DNA repair protein XRCC2, X-ray repair complementing defective repair in Chinese hamster cells 2.

Quality

Western Blot Analysis:
1:500 dilution of this antibody detected XRCC2 in 10 µg of a Jurkat cell lysate.

Target description

~32 kDa

Linkage

Replaces: MABE556

Physical form

Affinity purified polyclonal precipitated in ammonium sulfate.

Rabbit polyclonal precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Jurkat cell lysate, human lung, NIH/3T3, A431, HeLa cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable