07-1490-M

Anti-Calcineurin Antibody

from rabbit, purified by affinity chromatography

• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: rabbit
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, human
• species reactivity (predicted by homology): mammals
• technique(s): immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_000944.3
• UniProt accession no.: Q08209
• target post-translational modification: unmodified
• Gene Information: human ... PPP3CA(5530)

Description

General description

Calcineurin/PP2B is a serine/threonine protein phosphatase involved in the mediation of signal tranduction in a wide variety of cellular responses. Calcineurin/PP2B has been linked to Pmk1 MAP kinase and PI pathways, as well as to members of the small GTPase Rab/Ypt family and Type II myosin. Furthermore, calcineurin participates in the transduction of extracellular signals to the nucleus by dephosphorylation of members of the NFAT family of transcription factors. These linkages demonstrate calcineurin/PP2B′s multifunctional capabilities in the regulation of calcium signaling.

Specificity

Broad reactivity with mammal species is expected.

Recognizes both the catalytic A and regulatory B subunits of calcineurin, a calcium- and calmodulin-dependent protein phosphatase.

Immunogen

Purified bovine brain calcineurin.

Application

Detect Calcineurin using this Anti-Calcineurin Antibody validated for use in WB, IH(P) & IP.

Immunohistochemisty (paraffin): Representative testing from a previous lot. Calcineurin representative staining pattern/morphology in human vascular tissue. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select Detection with HRP-DAB.

Immunoprecipitation: 3 μL per reaction of a previous lot of this antibody was used in Immunoprecipitation.

Optimal working dilutions must be determined by end user

Research Category
Signaling

Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling

Quality

Routinely evaluated by Western Blot on PC3 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected PP2B B on 10 μg of PC3 lysates.

Target description

19 kDa

Linkage

Replaces: AB1694

Physical form

Affinity purified

Purified rabbit polyclonal IgG in buffer containing TBS and 40% glycerol as a cryopreservative.

Storage and Stability

Stable for 1 year at -20°C in undiluted aliquots from date of receipt; at this temperature the solution will remain unfrozen.
Handling Recommendations: Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

Analysis Note

Control
PC3 cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1