04-1572
Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8
clone 3E8, from rat
Synonym(s):
DNA-directed RNA polymerase II A, DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA polymerase II subunit B1, RNA-directed RNA
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rat
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3E8, monoclonal
species reactivity
mouse
species reactivity (predicted by homology)
human (based on 100% sequence homology)
technique(s)
ChIP: suitable
ELISA: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer5)
Gene Information
human ... POLR2B(5431)
General description
RNA polymerase II subunit B1 (RPB1) is the largest subunit of the RNA polymerase II complex. As a holoenzyme RNA polymerase II catalyzes transcription of eukaryotic DNA into RNA using the four ribonucleoside triphosphates as substrates. The RBP1 subunit, in combination with other polymerase subunits, forms a large central cleft that maintains contact between the active site of the enzyme, the DNA template, and the nascent RNA transcript. This subunit also contains a carboxy terminal domain (CTD) consisting of tandem heptapeptide repeats. Phosphorylation activates the RNA polymerase II beta subunit, allowing it to serve as an assembly platform for additional subunits that modulate initiation, elongation, termination and mRNA processing. In actively transcribing RNA polymerase ‘Ser-2’ and ‘Ser-5’ of the heptapeptide repeat are phosphorylated. Ser-7 is phosphorylated before initiation of transcription at promoter regions.
Specificity
This antibody recognizes RNA polymerase II subunit B1 at the CTD when phosphorylated at Ser5.
Immunogen
Epitope: Ser5
Ovalbumin-conjugated linear peptide corrresponding to human RNA polymerase subunit B1 CTD phosphorylated at Ser5.
Application
Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in ChIP. (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
RNA Metabolism & Binding Proteins
Transcription Factors
RNA Metabolism & Binding Proteins
Use Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8 (Rat Monoclonal Antibody) validated in WB, ELISA, ChIP to detect RNA polymerase II subunit B1 (phospho-CTD Ser-5).
Quality
Evaluated by Western Blot in γ-PPase untreated and treated NIH/3T3 cell lysates.
Western Blot Analysis: 1 µg/ml of this antibody detected RNA polymerase II CTD on 10 µg of γ-PPase untreated and treated NIH/3T3 cell lysates.
Western Blot Analysis: 1 µg/ml of this antibody detected RNA polymerase II CTD on 10 µg of γ-PPase untreated and treated NIH/3T3 cell lysates.
Target description
~ 220 kDa
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
γ-protein phosphatase (γ-Ppase) untreated and treated NIH/3T3 cell lysates
γ-protein phosphatase (γ-Ppase) untreated and treated NIH/3T3 cell lysates
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Yejun Wang et al.
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Activation-induced cytidine deaminase (AID) is required for initiation of Ig class switch recombination (CSR) and somatic hypermutation (SHM) of antibody genes during immune responses. AID has also been shown to induce chromosomal translocations, mutations, and DNA double-strand breaks (DSBs) involving
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Methods in enzymology, 551, 285-321 (2015-02-11)
Genome-wide analyses have revolutionized our ability to study the transcriptional regulation of circadian rhythms. The advent of next-generation sequencing methods has facilitated the use of two such technologies, ChIP-seq and RNA-seq. In this chapter, we describe detailed methods and protocols
Jean Mbogning et al.
Nucleic acids research, 43(20), 9766-9775 (2015-08-16)
Transcription by RNA polymerase II (RNAPII) is accompanied by a conserved pattern of histone modifications that plays important roles in regulating gene expression. The establishment of this pattern requires phosphorylation of both Rpb1 (the largest RNAPII subunit) and the elongation
Mitsunori Koga et al.
Nucleic acids research, 43(17), 8258-8267 (2015-07-24)
Phosphorylation of the C-terminal domain of the largest subunit of RNA polymerase II (Pol II), especially Ser2 and Ser5 residues, plays important roles in transcription and mRNA processing, including 5' end capping, splicing and 3' end processing. These phosphorylation events
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