04-1462
Anti-hnRNP F Antibody, clone 3H4
clone 3H4, from mouse
Synonym(s):
heterogeneous nuclear ribonucleoprotein F, nucleolin-like protein, HnRNP F protein, Nucleolin-like protein mcs94-1, mcs94-1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3H4, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... HNRNPF(3185)
General description
The hnRNP F protein is member of a subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs).
The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and associate with pre-mRNAs in the nucleus. These complexes are associated with pre-mRNA processing and other aspects of mRNA metabolism and transport. There are more than 20 different heterogeneous nuclear ribonucleoproteins (hnRNPs) associated with pre-mRNAs in the nucleus of mammalian cells. These different hnRNPs display a range of affinities for a variety of ribonucleotidehomopolymers and ssDNA sequences. In constrast, hnRNP F protein as well as hnRNP H (a highly related protein) appear to bind only to poly(rG). These unique RNA-binding properties, as well as the amino acid sequence and other data showing a distinct intranuclear localization of hnRNP F and hnRNP H suggest they are unique from other hnRNP and play an important role in the processing of RNAs containing guanosine-rich sequences.
The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and associate with pre-mRNAs in the nucleus. These complexes are associated with pre-mRNA processing and other aspects of mRNA metabolism and transport. There are more than 20 different heterogeneous nuclear ribonucleoproteins (hnRNPs) associated with pre-mRNAs in the nucleus of mammalian cells. These different hnRNPs display a range of affinities for a variety of ribonucleotidehomopolymers and ssDNA sequences. In constrast, hnRNP F protein as well as hnRNP H (a highly related protein) appear to bind only to poly(rG). These unique RNA-binding properties, as well as the amino acid sequence and other data showing a distinct intranuclear localization of hnRNP F and hnRNP H suggest they are unique from other hnRNP and play an important role in the processing of RNAs containing guanosine-rich sequences.
Specificity
This antibody recognizes hnRNP F.
Immunogen
Epitope: Unknown
Recombinant protein corresponding to human hnRNP F.
Application
Immunoprecipitation Analysis:
A representative lot was used by an independent laboratory for IP. (Matunis, M.J., et al. (1994). Nucleic Acids Res. 22(6):1059–1067.)
Immunofluorescence Analysis:
A representative lot was used by an independent laboratory in IF. (Matunis, M.J., et al. (1994). Nucleic Acids Res. 22(6):1059–1067.)
A representative lot was used by an independent laboratory for IP. (Matunis, M.J., et al. (1994). Nucleic Acids Res. 22(6):1059–1067.)
Immunofluorescence Analysis:
A representative lot was used by an independent laboratory in IF. (Matunis, M.J., et al. (1994). Nucleic Acids Res. 22(6):1059–1067.)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins
RNA Metabolism & Binding Proteins
Use Anti-hnRNP F Antibody, clone 3H4 (Mouse Monoclonal Antibody) validated in WB, IP, ICC to detect hnRNP F also known as heterogeneous nuclear ribonucleoprotein F.
Quality
Evaluated by Western Blot in K562 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected hnRNP F on 10 µg of K562 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected hnRNP F on 10 µg of K562 cell lysate.
Target description
Calculated: 46 kDa Observed: ~53 kDa
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
K562 cell lysate
K562 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Hui Miao et al.
Science advances, 8(51), eabq7289-eabq7289 (2022-12-24)
Understanding how long noncoding RNAs (lncRNAs) cooperate with splicing factors (SFs) in alternative splicing (AS) control is fundamental to human biology and disease. We show that metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), a well-documented AS-implicated lncRNA, regulates AS via two
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