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SAB4200521

Sigma-Aldrich

Anti-phospho-VASP [pThr278] antibody produced in rabbit

~0.1 mg/mL, affinity isolated antibody

Synonym(s):

Anti-Vasodilator-stimulated phosphoprotein

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~45 kDa

species reactivity

mouse, human, rat

concentration

~0.1 mg/mL

technique(s)

immunoprecipitation (IP): 40 μL using HEK-293T cells overexpressing human VASP
western blot: 1:5000-10000 using human platelets cell lysates

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pThr278)

Gene Information

human ... VASP(7408)
mouse ... Vasp(22323)
rat ... Vasp(361517)

General description

VASP is localized at highly dynamic membrane regions, focal adhesion sites, lamellipodia protrusions, filopodia tips, and along stress fibers. It is also localized at cell-matrix and cell-cell contacts.
Vasodilator-stimulated phosphoprotein (VASP) is located on human chromosome 19q13.32. It belongs to enabled (Ena) family of proteins and encodes a protein of 46 kDa. VASP has three domain organisation, that is, an N-terminal Ena/VASP (EVH1) homology domain, proline rich central domain and EVH2 actin binding domain.

Specificity

Anti-phospho-VASP [pThr278] specifically recognizes human VASP phosphorylated at Thr278.

Immunogen

synthetic peptide containing phosphorylated Thr278 of human VASP, conjugated to KLH. The corresponding sequence is identical in mouse and rat VASP.

Application

Anti-phospho-VASP [pThr278] antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Regulation of phosphorylation of Vasodilator-stimulated phosphoprotein (VASP) is observed during actin cytoskeleton formation. It promotes protrusion and propulsion speed and inhibits branching of actins. It plays a key role in the signal transduction pathway related to neutrophil spreading.
VASP plays an important role in cell motility and adhesion. This protein is also involved in adherens junction formation and stabilization in epithelial cells. VASP is a substrate for cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP)-dependent protein kinases. VASP is required for endothelial barrier function in vivo. Knockout of VASP proteins in mice leads to increased endothelial permeability causing fatal vascular leakage and hemorrhage during late embryonic development.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M Reinhard et al.
The EMBO journal, 14(8), 1583-1589 (1995-04-18)
Profilins are small proteins that form complexes with G-actin and phosphoinositides and are therefore considered to link the microfilament system to signal transduction pathways. In addition, they bind to poly-L-proline, but the biological significance of this interaction is not yet
Cloning of the VASP (vasodilator-stimulated phosphoprotein) genes in human and mouse: structure, sequence, and chromosomal localization
Zimmer M, et al.
Genomics, 36(2), 227-233 (1996)
Differential VASP phosphorylation controls remodeling of the actin cytoskeleton
Benz PM, et al.
Journal of Cell Science, 122(21), 3954-3965 (2009)
AMP-activated protein kinase impairs endothelial actin cytoskeleton assembly by phosphorylating vasodilator-stimulated phosphoprotein
Blume C, et al.
The Journal of Biological Chemistry, 282(7), 4601-4612 (2007)
C Haffner et al.
The EMBO journal, 14(1), 19-27 (1995-01-03)
The vasodilator-stimulated phosphoprotein (VASP), a substrate for cAMP- and cGMP-dependent protein kinases in vitro and in intact cells, is associated with actin filaments, focal adhesions and dynamic membrane regions. VASP, cloned here from human HL-60 and canine MDCK cells, is

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