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SAB1302339

Sigma-Aldrich

ANTI-MOUSE MLKL (C-TERM) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Mixed lineage kinase domain-like protein, Mlkl

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

54317 Da

species reactivity

mouse

technique(s)

western blot: 1:250-1:500

NCBI accession no.

NP_083281.1

UniProt accession no.

Q9D2Y4

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Mlkl(74568)

General description

The gene mixed lineage kinase domain-like protein (MLKL) is mapped to human chromosome 16q23. Activated MLKL is localized on the cell membrane.

Application

Anti mouse MLKL (C-TERM) antibody produced in rabbit has been used in immunoblotting.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Mixed lineage kinase domain-like protein (MLKL) primarily causes receptor-interacting protein (RIP) kinase–dependent necroptosis. However, during hepatitis, it results in programmed hepatocellular necrosis which is independent of RIPK3. MLKL also participates in endosomal trafficking and in the formation of extracellular vesicles.

Physical form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Necroptosis is a key pathogenic event in human and experimental murine models of non-alcoholic steatohepatitis.
Afonso M B, et al.
Clinical Science, 129(8), 721-739 (2015)
miRNA-21 ablation protects against liver injury and necroptosis in cholestasis.
Afonso M B, et al.
Cell Death and Differentiation, 25(5), 857?872-857?872 (2018)
MLKL activation triggers NLRP3-mediated processing and release of IL-1β independently of Gasdermin-D.
Gutierrez K D, et al.
Journal of Immunology, 198(5), 2156-2164 (2017)
Marta B Afonso et al.
Cell death and differentiation, 25(5), 857-872 (2017-12-13)
Inhibition of microRNA-21 (miR-21) prevents necroptosis in the mouse pancreas. Necroptosis contributes to hepatic necro-inflammation in the common bile duct ligation (BDL) murine model. We aimed to evaluate the role of miR-21 in mediating deleterious processes associated with cholestasis. Mechanistic
Jiali Cai et al.
Cell death & disease, 12(2), 140-140 (2021-02-06)
ABIN-1, also called TNIP1, is an ubiquitin-binding protein that serves an important role in suppressing RIPK1-independent apoptosis, necroptosis, and NF-κB activation. However, the involvement of ABIN-1 in the regulation of RIPK1-dependent apoptosis (RDA) is unknown. In this study, we found
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