immunoprecipitation (IP): 25-50 μg using Jurkat cell lysate microarray: suitable western blot: 2-4 μg/mL using whole cell extract of human acute T cell leukemia Jurkat cell line and of mouse fibroblasts NIH3T3 cell line.
ROCK2 is a serine/threonine kinase that regulates actin cytoskeletal framework. Absence of ROCK2 alleviates atherosclerosis, whereas its overexpression has been associated with hepatocellular carcinoma. Genetic mutations in ROCK2 has been linked to the risk of Behcet′s disease . Anti-ROCK-2 recognizes human and mouse ROCK-2 (160 kDa).
Immunogen
The sequence is highly conserved in Xenopus ROCK-2.
synthetic peptide corresponding to amino acids 1371-1388 located at the C-terminus of human ROCK-2, conjugated to KLH. This sequence is identical in rat, mouse and bovine ROCK-2.
Application
The antibody is suitable for use in immunoprecipitation (25-50 μg using Jurkat cell lysate), microarray and western blot (2-4 μg/mL using whole cell extract of human acute T cell leukemia Jurkat cell line and of mouse fibroblasts NIH3T3 cell line). The antibody may also be used for immunofluorescence (3-6 μg/ml using NIH3T3 cell line).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Rho-associated coiled-coil kinase (ROCK)2 targeting down-regulates autoimmune responses in animal models and patients, however the underlying molecular mechanism is still an enigma. We report that ROCK2 binds phosphorylated-STAT3 and its kinase activity controls the formation of ROCK2/STAT3/JAK2 complex and optimal
Rho-associated coiled-coil containing protein kinase 2 (Rock2) belongs to a family of serine/threonine kinases which are actived via interaction with Rho GTPases. Recently, overexpression of Rock2 has been demonstrated in human hepatocellular carcinoma (HCC), but the potential role of Rock2
ROCK2 Deficiency in Bone Marrow-Derived Cells Leads to Increased Cholesterol Efflux and Decreased Atherosclerosis.
International journal of molecular medicine, 36(3), 801-807 (2015-07-17)
The transformation of tunica albuginea-derived fibroblasts (TAFs) into myofibroblasts plays an important role in the pathological progress of Peyronie's disease (PD). However, no treatment which addresses this transformation is currently available. Estrogen has been shown to inhibit the progression of
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