PF0100
Protease Fluorescent Detection Kit
high sensitivity assay
Synonym(s):
Protease Assay Kit
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.26
Recommended Products
Application
Protease Fluorescent Detection Kit has been used:
- in the detection of proteolytic activity using casein based protease assay
- for testing the extracellular proteolytic activity of fungal strains
- for quantifying mitochondrial protease activity
- for measuring the protease production from soil solution
Suitability
Detection limit: approx. 50 ng trypsin
Quantity
Supplied with sufficient reagents for up to 200 one mL assays.
Supplied with sufficient reagents for up to 200 one mL assays.
The trichloroacetic acid (TCA) component is provided as 6.1 N TCA solution. 0.6 N TCA Solutions may be prepared by a 10-fold dilution of the desired volume of T0699 with ultrapure water. It is not necessary to dilute the entire volume of T0699 for immediate use of the kit.
The trichloroacetic acid (TCA) component is provided as 6.1 N TCA solution. 0.6 N TCA Solutions may be prepared by a 10-fold dilution of the desired volume of T0699 with ultrapure water. It is not necessary to dilute the entire volume of T0699 for immediate use of the kit.
Analysis Note
Tested for detection of all four proteolytic classes (serine, apartic, cysteine and metalloproteinases).
Kit Components Only
Product No.
Description
- Incubation buffer 25 mL
- FITC-casein solution 5 mL
- Assay buffer 200 mL
- Protease standard (trypsin) 20 μg
- FITC control 50 mg
- Trichloroacetic acid (TCA) solution 30 mL
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
8A - Combustible corrosive hazardous materials
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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