P2508

Proflavine hemisulfate salt hydrate

powder

• Synonym(s): 3,6-Diaminoacridine hemisulfate salt
• Empirical Formula (Hill Notation): C₁₃H₁₁N₃ · 0.5 H₂SO₄ · xH₂O
• CAS Number: 1811-28-5
• Molecular Weight: 258.29 (anhydrous basis)
• Beilstein: 3921806
• EC Number: 217-320-3
• MDL number: MFCD00149650
• UNSPSC Code: 12171500
• PubChem Substance ID: 24898329
• NACRES: NA.47

Properties

• form: powder
• solubility: H₂O: 10 mg/mL
• fluorescence: λ_ex 444 nm; λ_em 508 nm (0.01M HCl)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [H]O[H].OS(O)(=O)=O.Nc1ccc2cc3ccc(N)cc3nc2c1.Nc4ccc5cc6ccc(N)cc6nc5c4
• InChI: 1S/2C13H11N3.H2O4S.H2O/c2*14-10-3-1-8-5-9-2-4-11(15)7-13(9)16-12(8)6-10;1-5(2,3)4;/h2*1-7H,14-15H2;(H2,1,2,3,4);1H2
• InChI key: DTRLDKDFCPUGCA-UHFFFAOYSA-N

Description

General description

Proflavine is a small, amphipathic, acridine-derived fluorescent dye.Proflavine hemisulfate is used as a rapid stain for cytological studies of biological specimens. It stains cytoplasmic structures and nuclei. Proflavine has the capability to intercalate double stranded DNA.

Application

Proflavine hemisulfate salt hydrate has been used to stain cells of the 1483 cell line. It has also been used as a calibrating dye in fluorescence resonance energy transfer (FRET) analysis.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H302,H315,H319,H335
• Precautionary Statements: P261 - P264 - P271 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves