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Merck

P1406

Protein A–Agarose

lyophilized powder

Synonym(s):

Protein A resin

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56
MDL number:
MFCD00165563

Key Documents

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Product Name

Protein A–Agarose, lyophilized powder

form

lyophilized powder

extent of labeling

~2 mg per mL

matrix

Cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

1 atom

capacity

≥15 mg/mL binding capacity (human IgG)

storage temp.

−20°C

Quality Level

200

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This Item
P7700P1925P6649
Protein A–Agarose lyophilized powder

Millipore

P1406

Protein A–Agarose

Protein G–Agarose lyophilized powder, Contains lactose stabilizers that must be removed prior to use.

Millipore

P7700

Protein G–Agarose

Protein A–Agarose macrobeads aqueous suspension

Millipore

P1925

Protein A–Agarose macrobeads

Protein A–Sepharose™ 6MB aqueous ethanol suspension

Millipore

P6649

Protein A–Sepharose™ 6MB

form

lyophilized powder

form

lyophilized powder

form

aqueous suspension

form

aqueous ethanol suspension

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

matrix

Cross-linked 4% beaded agarose

matrix

cross-linked 4% beaded agarose

matrix

-

matrix

-

extent of labeling

~2 mg per mL

extent of labeling

-

extent of labeling

~6 mg per mL

extent of labeling

~1 mg per mL

matrix activation

cyanogen bromide

matrix activation

cyanogen bromide

matrix activation

cyanogen bromide

matrix activation

cyanogen bromide

Application

Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer. Protein A–Agarose has been used as an immobilized biosensor.[1] It has also been used for immunoprecipitation.[2]

Physical form

Supplied as lyophilized powder stabilized with lactose.

Preparation Note

Swelling: 1 g swells to 3-6 ml

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

hcodes

H314,H335

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B - STOT SE 3

target_organs

Respiratory system

Storage Class

8A - Combustible corrosive hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SLCQ1494
SLCJ3404
SLBW2466
SLBJ2711V
SLBB3875V

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L G Reddy et al.
The Journal of biological chemistry, 270(16), 9390-9397 (1995-04-21)
Phospholamban (PLB) is a small, transmembrane protein that resides in the cardiac sarcoplasmic reticulum (SR) and regulates the activity of Ca(2+)-ATPase in response to beta-adrenergic stimulation. We have used the baculovirus expression system in Sf21 cells to express milligram quantities
A Schmidt et al.
European journal of biochemistry, 248(2), 543-549 (1997-11-05)
Basic fibroblast growth factor (bFGF), a potent mitogen for arterial smooth muscle cells, has been shown to play a fundamental role in the pathogenesis of arteriosclerosis and restenosis by stimulating the proliferation of vascular smooth muscle cells. We found that
P Libby et al.
The American journal of pathology, 124(2), 179-185 (1986-08-01)
Interleukin 1 (IL-1) can induce potentially pathogenic functions of vascular endothelial cells. This mediator was formerly thought to be produced primarily by activated macrophages. We report here that bacterial endotoxin and recombinant human tumor necrosis factor cause accumulation of IL-1
Histone deacetylase inhibition enhances in-vivo bone regeneration induced by human periodontal ligament cells
Huynh NN, et al.
Bone (2017)
P K Schick et al.
Blood, 87(5), 1817-1823 (1996-03-01)
There are several species of alternatively spliced fibronectin (FN). One of these, FN EIIIB, is primarily present in embryonic and in proliferating and migrating cells and is believed to be important for cell maturation. We have studied the synthesis, localization
View All Related Papers

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