I2771
ITS+3 Liquid Media Supplement (100×)
liquid, sterile-filtered, BioReagent, suitable for cell culture
Synonym(s):
ITS Supplement
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About This Item
UNSPSC Code:
12352205
NACRES:
NA.75
Recommended Products
sterility
sterile-filtered
product line
BioReagent
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
shipped in
ambient
storage temp.
2-8°C
General description
ITS+3 liquid media supplement is a mixture of insulin, human transferrin (partially iron-saturated), sodium selenite, linoleic acid, and bovine serum albumin (BSA). It is suitable for serum-free cell culture applications.
Application
ITS+3 Liquid Media Supplement (100×) has been used as a media supplement:
- to support the growth of human adipose stem cells (ASC)
- to culture porcine intervertebral disc (IVD) cells and tissues
- to culture cartilage endplate (CEP) cells
Biochem/physiol Actions
- Insulin: a polypeptide hormone that promotes glucose and amino acid uptake and may produce a mitogenic effect.
- Transferrin: an iron-transport protein. Iron is an essential trace element but can be toxic in its free form. To nourish cells in culture, iron is supplied bound to transferrin in serum.
- Selenium: an essential trace element present in serum.
Components
Contains 1.0 mg/ml bovine insulin, 0.55 mg/ml human transferrin (substantially iron-free), 0.5 μg/ml sodium selenite, 470 μg/ml linoleic acid, 470 μg/ml oleic acid and 50 mg/ml bovine serum albumin at the 100x concentration. Contains 2 moles each of linoleic acid and oleic acid per mole of albumin.
Preparation Note
Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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