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GE28-9187-79

MBPTrap™ HP

Cytiva 28-9187-79, pack of 1 × 5 mL

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

ligand

dextrin

packaging

pack of 1 × 5 mL

manufacturer/tradename

Cytiva 28-9187-79

storage condition

(20% Ehtanol)

parameter

<20 mL/min flow rate
42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

2-13

working range

>7

capacity

~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium)

storage temp.

2-8°C

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General description

MBPTrap™ HP columns prepacked with Dextrin Sepharose™ High Performance provide convenient, high binding capacity, affinity purification of recombinant proteins tagged with maltose binding protein (MBP). This robust and stable Sepharose High Performance based resin enables elution of target protein in narrow peaks, which minimizes the need for further concentration steps.

Purification is performed under physiological conditions and the mild elution preserves the activity of the target protein. The mild conditions even allow purification of intact protein complexes. Due to the high specificity of the binding, very high purity of the eluted protein is achieved in just one step. Dextrin Sepharose High Performance and MBPTrap HP are easily regenerated using 0.5 M NaOH.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Features and Benefits

  • HiTrap™ columns prepacked with Dextrin Sepharose™ High Performance medium.
  • Highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes.
  • Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH.
  • Physiological conditions and mild elution preserve the activity of the target protein.
  • Convenient, time-saving operation and reproducible resultsLab Purification using the affinity to the MBP tag.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

MBPTrap is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Anh Hoang Le et al.
The Journal of cell biology, 220(9) (2021-06-25)
The Scar/WAVE complex drives actin nucleation during cell migration. Interestingly, the same complex is important in forming membrane ruffles during macropinocytosis, a process mediating nutrient uptake and membrane receptor trafficking. Mammalian CYRI-B is a recently described negative regulator of the

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