GE28-9031-32
Protein A High Performance Spintrap™
Cytiva 28-9031-32, pack of 16 columns
Synonym(s):
High Performance Spintrap, Protein A Spintrap
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About This Item
UNSPSC Code:
41106500
NACRES:
NA.25
Recommended Products
ligand
native protein A
feature
wetted part: no
packaging
pack of 16 columns
manufacturer/tradename
Cytiva 28-9031-32
storage condition
(20% Ethanol)
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
2-10
working range
3-9
capacity
>1 mg binding capacity(human IgG well)
storage temp.
2-8°C
General description
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
protein A HP SpinTrap™ are designed for the enrichment of proteins of interest, immunoprecipitation and the purification of monoclonal and polyclonal antibodies from cell lysates and biological fluids. Contain protein A Sepharose™ HP.
Application
Protein A Sepharose™ HP has high affinity for the Fc region of IgG from a variety of species. The two options in the Protein enrichment protocol allow the attached antigen (Protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.
There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.
There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.
Features and Benefits
- For simple, small-scale antibody purification or enrichment of specific Proteins in prepacked, single-use spin columns that are ready to use.
- Classic and cross-link protocols provide flexibility.
- Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
- Fast binding kinetics and high capacity provide high yield.
- Prepacked with Protein A Sepharose™ High Performance for coupling of antibodies of IgG subclasses.
- Easy scale-up with HiTrap® Protein A HP prepacked columns.
Storage and Stability
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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