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GE17-5113-01

Streptavidin Sepharose High Performance

Cytiva 17-5113-01, pack of 5 mL

Synonym(s):

affinity chromatography medium, high performance affinity resin, biotinylated proteins purification

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

packaging

pack of 5 mL

manufacturer/tradename

Cytiva 17-5113-01

storage condition

(20% Ethanol)

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning in place

2-10.5

working range

4-9

capacity

binding capacity(>300 nmol biotin/ml medium 6 mg biotinylated BSA/ml medium)

storage temp.

2-8°C

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General description

Streptavidin Sepharose High Performance is an affinity chromatography medium designed for fast and reliable, high resolution purification of biotinylated biomolecules.

Purified Streptavidin isolated from Streptomyces avidinii is immobilised on Sepharose High Performance. The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability. The immobilised streptavidin binds biotin and biotinylated substances through affinity chromatography.

Features and Benefits

  • Streptavidin immobilized to Sepharose High Performance for purification of biotinylated compounds
  • Extremely useful for exploiting either the strong interactions of biotin and streptavidin or the somewhat weaker interactions of 2-iminobiotin and streptavidin.
  • 34 μm bead size for improved resolution

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jérôme Zervudacki et al.
The EMBO journal, 37(14) (2018-06-07)
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We used BioID, a proximity-dependent biotinylation assay with 100 mitochondrial baits from all mitochondrial sub-compartments, to create a high-resolution human mitochondrial proximity interaction network. We identified 1,465 proteins, producing 15,626 unique high-confidence proximity interactions. Of these, 528 proteins were previously
Sarah van Veen et al.
Nature, 578(7795), 419-424 (2020-01-31)
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