G1890
Porcine Gelatin
from porcine skin, Type A, powder, gel strength ~300 g Bloom, suitable for electrophoresis and cell culture
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product name
Gelatin from porcine skin, powder, gel strength ~300 g Bloom, Type A, BioReagent, suitable for electrophoresis, suitable for cell culture
biological source
Porcine skin
sterility
sterile
type
Type A
product line
BioReagent
form
powder
mol wt
50—100 kDa
packaging
pkg of 1 kg
pkg of 100 g
pkg of 500 g
concentration
70—90% Biuret
technique(s)
cell culture | mammalian: suitable
electrophoresis: suitable
surface coverage
100‑200 μg/cm2
solubility
H2O: soluble 50 mg/mL, clear to hazy, faintly yellow
shipped in
ambient
storage temp.
room temp
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General description
Gelatin from porcine skin is generated from the acidic digestion of collagen and is referred as type A. Porcine gelatin comprises majorly glycine, proline and hydroxyproline. Gelatin takes up random coil structure after digestion from the triple helical collagen. It differs from type B bovine gelatin at the N-terminal sequence.
Application
Gelatin has been used in many applications. It has been used in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has been used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, gelatin can be used as a suspending and encapsulating agent, among other applications. This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.
Features and Benefits
- Sterile gelatin sourced from porcine skin.
- Free from proteases.
- In the form of gelatin powder for convenient and easy handling.
- Versatile for use in cell culture and electrophoresis techniques.
- Provides adequate surface coverage for various applications.
Components
Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.
Caution
Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.
Preparation Note
This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of approximately 300.
Storage Class Code
11 - Combustible Solids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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