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CHOGS

CHOZN® ZFN-Modified CHO Cell Line

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NACRES:
NA.75
UNSPSC Code:
12352200

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description

CHOZN® GS-/- ZFN-modified CHO cell line
glutamine synthetase knockout cell line

solubility

DMSO: 5 mg/mL, clear

shipped in

liquid nitrogen

storage temp.

−196°C

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This Item
CHODHFRCHOK1SCC494
CHOZN® ZFN-Modified CHO Cell Line

SAFC

CHOGS

CHOZN® ZFN-Modified CHO Cell Line

CHOZN® ZFN-Modified CHO Cell Line

SAFC

CHODHFR

CHOZN® ZFN-Modified CHO Cell Line

CHOZN® CHO K1 Host Cell Line Suspension-adapted in CD media

SAFC

CHOK1

CHOZN® CHO K1 Host Cell Line

Mouse OP9-DL4 Delta-like 4 Notch Ligand Cell Line

Sigma-Aldrich

SCC494

Mouse OP9-DL4 Delta-like 4 Notch Ligand Cell Line

shipped in

liquid nitrogen

shipped in

dry ice

shipped in

dry ice

shipped in

liquid nitrogen

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

description

CHOZN® GS-/- ZFN-modified CHO cell line

description

CHOZN® DHFR-/- ZFN-modified CHO cell line, dihydrofolate reductase knockout cell line

description

CHOK1 cell line derived from ECACC CHO K1

description

-

solubility

DMSO: 5 mg/mL, clear

solubility

-

solubility

-

solubility

-

Biochem/physiol Actions

CHOZN cell lines are all subclones of the parental CHO-K1 cell line originated by Puck in 1957.

General description

CHOZN cell lines were created utilizing Sigma′s proprietary CompoZr Zinc Finger Nuclease (ZFN) technology to create genetic modifications in parental CHO-K1 cells. Cells are banked in chemically defined, animal component free EX-CELL CD CHO Fusion media
ZFNs are a class of engineered DNA-binding proteins and causes a double-strand break. This double-strand break is necessary for site-specific mutagenesis mediated DNA repair. It facilitates targeted genome editing by binding to a user-specified locus.[1] Glutamine synthetase (GS) is one of the most commonly used selectable markers in the biopharmaceutical industry. Glutamine is an essential amino acid for cellular growth.[2] The GS enzyme is responsible for the conversion of glutamate into glutamine.[3] Without this enzymatic activity, cells can no longer synthesize glutamine endogenously and if glutamine is not supplemented in the culture media, the cells will die.

Legal Information

All CHOZN cell lines are sold under license from Sigma Aldrich. A copy of the research license is provided with purchase of the CHOZN cell lines.
CHOZN is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

This product is shipped in liquid nitrogen dry vapor shippers.
Liquid nitrogen dry vapor shipper fee (up to a maximum of $125) and freight not included in price of product.
Liquid nitrogen dry vapor shipper is not disposable and should be returned after receipt. A $450 liquid nitrogen dry vapor shipper fee will be charged if not returned within 1 week of delivery.
Learn more about liquid nitrogen dry vapor shipper

Physical form

CHOZN cell lines are provided to customers in vials containing >7.5e6 cells/mL

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
2113545207
2113545207
2113-53667
051M0924

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Q's next: the diverse functions of glutamine in metabolism, cell biology and cancer.
DeBerardinis R J and Cheng T
Oncogene, 29(3), 313-313 (2010)
The metabolic profile of tumors depends on both the responsible genetic lesion and tissue type.
Yuneva M O, et al.
Cell Metabolism, 15(2), 157-170 (2012)
Cytotoxic effects during knock out of multiple porcine endogenous retrovirus (PERV) sequences in the pig genome by zinc finger nucleases (ZFN).
Semaan M, et al.
PLoS ONE, 10(4), e0122059-e0122059 (2015)

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