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CELLMM2

Millipore

FLAG® M Purification Kit

For Mammalian expression systems.

Synonym(s):

Anti-ddddk, Anti-dykddddk

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

Quality Level

200

technique(s)

protein extraction: suitable

shipped in

wet ice

storage temp.

−20°C

Application

The FLAG Purification Kit utilizes CelLytic™ Reagents for rapid and efficient cell lysis and protein extraction and ANTI-FLAG® M2 affinity gel for affinity purification of active FLAG fusion proteins.

Learn more product details in our FLAG® application portal.

Features and Benefits

  • Includes ready to use reagents, columns, and a detailed protocol for affinity purification of FLAG fusion proteins.
  • ANTI-FLAG M2 Affinity Gel allows efficient binding of FLAG fusion proteins without the need for preliminary steps and calibrations.
  • Two alternatives for efficient elution conditions (by acidic conditions or by competition with FLAG peptide).

Packaging

Sufficient for 3-5 uses of 1-ml affinity purification column.

Other Notes

Kit contains CelLytic™ M for cell lysis and protein extraction from Mammalian cells.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
CelLytic is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

Product No.
Description
SDS
  • C2978CelLytic™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.SDS
  • SAE0194Purified 3xFLAG® peptide, ≥95% (HPLC), lyophilized powderSDS
  • A2220ANTI-FLAG® M2 Affinity Gel, purified immunoglobulin, buffered aqueous glycerol solutionSDS
  • C2103Chromatography columns, general-purpose, volume 10 mL, Overall H 5 in.SDS

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nitric oxide (NO) has been thought to regulate the immune system through S nitrosylation of the transcriptional factor NF-kappaB. However, regulatory effects of NO on innate immune responses are unclear. Here, we report that NO has a capability to control
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Retinoic acid receptors (RARs) belong to the nuclear receptor superfamily. The mechanism of ligand-dependent activation of RARs is well known. The effect of protein phosphorylation on the activity of RARs has also been demonstrated. However, it is unclear whether other
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