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product name
T24/83, 85061107
biological source
human bladder
growth mode
Adherent
karyotype
Hypodiploidy to hypopentaploidy
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human bladder carcinoma
Cell Line Description
Derivative of the original T24 human bladder carcinoma cell line. Tumours are not produced in nude mice but tumour formation has been noted in hamster cheek pouches.
Culture Medium
McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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