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C3735

Sigma-Aldrich

Cytochrome P450 human

1A1 Isozyme Microsomes, with P450 Reductase, recombinant, expressed in baculovirus infected insect cells (BTI-TN-5B1-4)

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About This Item

Enzyme Commission number:
MDL number:
UNSPSC Code:
12161501
NACRES:
NA.47

biological source

human

Quality Level

recombinant

expressed in baculovirus infected insect cells (BTI-TN-5B1-4)

form

solution

specific activity

≥4 units/pmol enzyme

mol wt

45-60 kDa

packaging

vial of 0.5 nmol

technique(s)

activity assay: suitable

solubility

water: soluble

suitability

suitable for molecular biology

UniProt accession no.

application(s)

cell analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CYP1A1(1543)

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General description

Research area: Immuno and CKS

Cytochrome P450 (CYP) enzymes are a family of enzymes that are encoded by the P450 genes. These enzymes are membrane bound hemoproteins. that are mainly found in the liver’s endoplasmic reticulum.

Biochem/physiol Actions

Cytochrome P450 is a heterogeneous family of isozymes whose primary function is to oxidize small molecules, both as a function of intermediary metabolism (e.g., fatty acids) and to detoxify exogenous compounds (drugs or toxins). Some isoforms have narrow substrate specificity, while others are promiscuous. The CYP1A1 isoform catalyzes 7-deethylation of ethoxyresorufin. Cytochrome P450 (CYP) plays an important role in detoxifying xenobiotics, cellular metabolism and homeostasis. One of the main mechanisms of drug-drug interactions is the induction or inhibition of these enzymes. CYP enzymes are transcriptionally activated by a variety of xenobiotics and by endogenous substrates via receptor-dependent pathways. Inhibition of these enzymes is a major factor in metabolism-based drug-drug interactions, and many chemotherapeutic medications can cause drug interactions by either inhibiting or inducing the cytochrome p450 enzyme system.

Physical form

Solution in 100 mM potassium phosphate buffer, pH 7.4.

Preparation Note

Microsomes containing human CYP1A1 and recombinant human NADPH-P450 reductase.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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