91414
Tris buffered saline with Tween® 20
tablet (for 500 mL), pH 7.6, BioUltra, with Tween® 20
Synonym(s):
TBS, TBST
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product name
Tris buffered saline with Tween® 20, BioUltra, tablet (for 500 mL), pH 7.6
product line
BioUltra
Quality Level
form
tablet (for 500 mL)
pH
7.6
solubility
water: 2 tablet/liter, clear, colorless
application(s)
diagnostic assay manufacturing
Application
Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris buffered saline with Tween 20, pH 7.6 is used as a washing buffer in assays such as enzyme-linked immunosorbent assays.
Reconstitution
1 tablet dissolved in 500 mL of deionized water yields:
0.15 M sodium chloride, 0.050 m TRIS-HCl buffer; 0..05% Tween® 20;
pH 7.6 at 25°C
0.15 M sodium chloride, 0.050 m TRIS-HCl buffer; 0..05% Tween® 20;
pH 7.6 at 25°C
Legal Information
TWEEN is a registered trademark of Croda International PLC
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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