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Sigma-Aldrich

Atto 490LS NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

UNSPSC Code:
12352108
NACRES:
NA.32

product line

BioReagent

Quality Level

Assay

≥90% (HPLC)
≥90% (degree of coupling)

form

solid

mol wt

Mw 793 g/mol

manufacturer/tradename

ATTO-TEC GmbH

λ

in PBS, pH 7.4

UV absorption

λ: 491-497 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 490LS is a new fluorescent label featuring an extraordinary large Stokes-Shift of 165 nm. Thus the emission spectrum is almost completely separated from its absorption spectrum, making the dye highly suitable for multiplexing experiments, in particular in combination with Atto 488 and Atto 514. Atto 490LS is very hydrophillic and shows excellent water solubility. The dye exhibits a relatively high fluorescence quantum yield, which is only slightly reduced after conjugation to biomolecules, e.g. proteins, even at high degrees of labeling (DOL). Atto 490LS is an anionic dye. After conjugation to a substrate the dye carries a net electrical charge of -1.

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Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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