78102

Anti-Mouse IgG - Atto 633 antibody produced in goat

~1 mg/mL IgG, affinity isolated antibody

• Synonym(s): Anti-Mouse IgG−Atto 633 antibody produced in goat, Atto 633 [goat-Anti-mouse IgG]
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: goat
• Quality Level: 100
• conjugate: Atto 633 conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• contains: 50% glycerol as stabilizer
• species reactivity: mouse
• concentration: ~1 mg/mL IgG
• fluorescence: λ_ex 636 nm; λ_em 650 nm in PBS
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Anti-mouse IgG (whole molecule) is purified from goat anti-mouse IgG antiserum to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity. Goat anti-mouse is conjugated to Atto 633 NHS and purified by gel permeation chromatography and dialysis to remove unbound dye. Atto 633-goat anti mouse IgG associates with mouse IgGs.

Immunogen

purified mouse IgG (whole molecule).

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spect

Physical form

Solution in phosphate buffered saline and 5 mM sodium azide, pH 7.5, with glycerol 1:1

Analysis Note

Unconjugated dye ≤5% of total fluorescence

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Hazard Statements: H412
• Precautionary Statements: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves