When reconstituted in 50 mM Tris-HCl, pH 8.0, the solution is stable for at least one month at 2–8 °C. Similar stability would be expected with other buffers at the appropriate pH.
62288
Lipase B Candida antarctica, recombinant from Aspergillus oryzae
powder, beige, ~9 U/mg
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recombinant
expressed in Aspergillus oryzae
Quality Level
form
powder
specific activity
~9 U/mg
mol wt
33 kDa
color
beige
storage temp.
2-8°C
InChI
1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
InChI key
QWZUIMCIEOCSJF-CHHCPSLASA-N
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General description
Application
Lipase B Candida antarctica, recombinant from Aspergillus oryzae has been used:
- as a standard to characterize the enzymatic properties of D5-CalB[3]
- as an efficient biocatalyst to start the reaction to obtain (R)-ester via esterification of racemic secondary alcohol[4]
- to investigate a “green” recycling route for polybutylene succinate (PBS) based on reactive extrusion[5]
- to compare the esterification yield with adsorbed CaLB (aCaLB) and covalently immobilized CaLB (cCaLB)[6]
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.
Biochem/physiol Actions
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Candida antarctica lipase B (CALB) possesses wide substrate specificity, high activity and high enantioselectivity, hence it is considered as a major enzyme in biotechnology. It also has the capability to perform in aqueous and non-aqueous reaction environments. CALB is used in transesterification, kinetic resolution and polymerization reactions.[2]
Candida antarctica lipase B (CALB) possesses wide substrate specificity, high activity and high enantioselectivity, hence it is considered as a major enzyme in biotechnology. It also has the capability to perform in aqueous and non-aqueous reaction environments. CALB is used in transesterification, kinetic resolution and polymerization reactions.[2]
Lipase B from Candida antarctica has been shown to be an effective catalyst for the synthesis of esters of ethyl D-glucopyranoside from fatty acids larger than octanoic acid.[7] It has also been found to catalyze a wide variety of organic reactions including many different regio- and enantio-selective syntheses.[8]
Unit Definition
1 U corresponds to the amount of enzyme which liberates 1 μmol butyric acid per minute at pH 8.0 and 40°C (tributyrin, Cat. No. 91010, as substrate)
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Customers Also Viewed
Wenjie Yang et al.
JACS Au, 1(12), 2172-2181 (2022-01-04)
The immobilization of enzymes in metal-organic frameworks (MOFs) with preserved biofunctionality paves a promising way to solve problems regarding the stability and reusability of enzymes. However, the rational design of MOF-based biocomposites remains a considerable challenge as very little is
Simple amino acid tags improve both expression and secretion of Candida antarctica lipase B in recombinant Escherichia coli
Kim S K, et al.
Biotechnology and Bioengineering, 112(2), 346-355 (2015)
Structural behavior of Candida antarctica lipase B in water and supercritical carbon dioxide: A molecular dynamic simulation study
Housaindokht M R, et al.
Journal of Supercritical Fluids, 63, 180-186 (2012)
Sun-Ki Kim et al.
Biotechnology and bioengineering, 112(2), 346-355 (2014-09-04)
Escherichia coli is the best-established microbial host strain for production of proteins and chemicals, but has a weakness for not secreting high amounts of active heterologous proteins to the extracellular culture medium, of which origins belong to whether prokaryotes or
A green method for polybutylene succinate recycling: Depolymerization catalyzed by lipase B from Candida antarctica during reactive extrusion
Jbilou F, et al.
European Polymer Journal, 68, 207-215 (2015)
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After dissolution of Lipase B in 0.05M potassium phosphate buffer (pH=7.5), at what temperature should it be stored?
1 answer-
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How pure is Product 62288, Lipase B Candida antarctica, recombinant from Aspergillus oryzae?
1 answer-
Purity is not determined for this product. As is the case with many of our enzymes, the product is assayed for enzymatic activity, not for purity.
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What solvent can Lipase B Candida antarctica, recombinant from Aspergillus oryzae, Product 62288, be dissolved in?
1 answer-
Lipase B is expected to be soluble in water. We are able to dissolve the enzyme in water for the enzymatic assay. We also have tested the solubility of Lipase B in 0.05M potassium phosphate buffer solution (pH=7.5) during quality control at a concentration of 10mg/mL. According to the chemicals encyclopedia published by the Royal Society of Chemistry, almost all organic solvents decrease lipase activity, with the exception of petroleum ether.
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Is Product 62288, Lipase B Candida antarctica, recombinant from Aspergillus oryzae, lyophilized?
1 answer-
This lipase is a lyophilisate and may contain Gum arabic for adjustment of activity. The amount of Gum arabic in each vial is lot dependent.
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What is Product 62288, Lipase B Candida antarctica, recombinant from Aspergillus oryzae, soluble in?
1 answer-
Lipase B is expected to be soluble in water. We are able to dissolve the enzyme in water for the enzymatic assay. We also have tested the solubility of Lipase B in 0.05 M potassium phosphate buffer solution (pH=7.5) during quality control at a concentration of 10 mg/mL. According to the chemicals encyclopedia published by the Royal Society of Chemistry, almost all organic solvents decrease lipase activity, with the exception of petroleum ether.
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What is the molecular weight of Lipase B Candida antarctica, recombinant from Aspergillus oryzae, Product 62288?
1 answer-
Unfortunately, molecular weight information is unavailable at this time.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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