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E7889

Sigma-Aldrich

Ethylenediaminetetraacetic acid disodium salt solution

for molecular biology, 0.5 M in H2O, DNase, RNase, NICKase and protease, none detected

Synonym(s):

(Ethylenedinitrilo)tetraacetic acid disodium salt, EDTA disodium salt, EDTA-Na2

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About This Item

Linear Formula:
[-CH2N(CH2CO2Na)CH2CO2H]2
CAS Number:
139-33-3
Molecular Weight:
336.21
Beilstein:
3822669
MDL number:
MFCD00070672
UNSPSC Code:
12352107
PubChem Substance ID:
24894660
NACRES:
NA.31

grade

for molecular biology

Quality Level

300

sterility

0.2 μm filtered

form

liquid

reaction suitability

reagent type: chelator

concentration

0.5 M in H2O

pH

7.5-8.5

suitability

suitable for molecular biology

application(s)

microbiology

foreign activity

DNase, RNase, NICKase and protease, none detected

SMILES string

[Na+].[Na+].OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;/q;2*+1/p-2

InChI key

ZGTMUACCHSMWAC-UHFFFAOYSA-L

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General description

Ethylenediaminetetraacetic acid (EDTA) is a polyprotic acid. The four carboxylic acid and two amine groups with lone-pair electrons of EDTA can chelate calcium and several other metal ions.
Ethylenediaminetetraacetic acid disodium salt solution is a chelating agent suitable for molecular biology applications.

Application

Ethylenediaminetetraacetic acid disodium salt solution has been used:
  • in Tris hydrochloric acid (HCl) for digesting proteinase-K to determine the amount of DNA in the corneal sample
  • with papain to digest corneal samples for the quantification of glycosaminoglycan (GAG) content
  • in pretreatment for detaching epithelial cells grown to 80% confluency
  • to prepare stock solutions for lysis buffer
  • in the preparation of urea lysis buffer

Features and Benefits

molecular biology grade

Preparation Note

Prepared in 18 megohm water with Molecular Biology Reagent EDTA (E 5134)

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Pictograms

Health hazard
GHS08

Signal Word

Warning

Hazard Statements

H373

Precautionary Statements

P260 - P314 - P501

Hazard Classifications

STOT RE 2 Inhalation

Target Organs

Respiratory Tract

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000391927
0000384159
0000387324
0000392905
0000390872

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