FIBRP-RO
Roche
Fibronectin (pure)
from human plasma, >95% (SDS-PAGE), lyophilized (clear, colorless solution after reconstitution)
Synonym(s):
Fibronectin
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About This Item
MDL number:
UNSPSC Code:
12352202
Recommended Products
biological source
human plasma
Quality Level
sterility
non-sterile; 0.2 μm filtered
Assay
>95% (SDS-PAGE)
form
lyophilized (clear, colorless solution after reconstitution)
mol wt
440 kDa
packaging
pkg of 1 mg (11051407001)
pkg of 5 mg (11080938001)
manufacturer/tradename
Roche
technique(s)
cell culture | mammalian: suitable
UniProt accession no.
storage temp.
2-8°C
Gene Information
human ... FN1(2335)
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General description
Fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix.
Specificity
Active on most mammalian cells
Biological activity: Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage).
Biological activity: Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage).
Application
Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes. It has been used for the cell adhesion assay.
Sequence
Human plasma fibronectin consists of two similar polypeptide chains ( α-, β-chain, each of 220 kDa), connected by two disulfide bridges.
Physical form
Lyophilizate containing 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin
Preparation Note
Working concentration: 5μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.
Reconstitution
Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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