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NA02A

Sigma-Aldrich

Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate

suspension (Liquid), clone K121, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

K121, monoclonal

form

suspension (Liquid)

contains

≤0.1% sodium azide as preservative

species reactivity

human, mouse, rat

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Conjugated to agarose. Recognizes the exposed 5′ cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs).
Recognizes the exposed 5′ cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs). Also recognizes 7-methylguanosine-capped mRNA.
This Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate is validated for use in Affinity Purification, IP, IP, ICC, Paraffin Sections for the detection of 2,2,7-Trimethylguanosine.

Immunogen

2,2,7-trimethylguanosine

Application

Affinity Purification (see application references)

Immunoprecipitation (15 µl beads/sample)

Immunoprecipitation (1-2 µg/ml; see application references)

Immunocytochemistry (use Cat. No. NA02)

Paraffin Sections (use Cat. No. NA02)

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS.

Analysis Note

Negative Control
Transfer RNA (tRNA)
Positive Control
Heterogenous nuclear RNA (hnRNA)

Other Notes

Also recognizes 7-methylguanosine-capped mRNA. 2,2,7-trimethylguanosine (Ab-1) is human, mouse, and rat reactive. This antibody reacts with the exposed 5′ cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography. All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual sample types.
Casciola-Rosen, L.A., et al. 1994. J. Biol. Chem.269, 30757-30760.
Krainer, A.R. 1988. Nuc. Acids Res.16, 9415.
Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71.
Reddy, R. and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
Krainer, A.R. and Maniatis, T. 1985. Cell42, 725.
Kramer, A., et al. 1984. Cell38, 299.
Lerner, M.R., et al. 1980. Nature283, 220.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Kyle A Nilson et al.
Molecular cell, 59(4), 576-587 (2015-08-11)
The Cdk7 subunit of TFIIH phosphorylates RNA polymerase II (Pol II) during initiation, and, while recent studies show that inhibition of human Cdk7 negatively influences transcription, the mechanisms involved are unclear. Using in vitro transcription with nuclear extract, we demonstrate that
Philippe Ganot et al.
Molecular and cellular biology, 24(17), 7795-7805 (2004-08-18)
trans splicing of a spliced-leader RNA (SL RNA) to the 5' ends of mRNAs has been shown to have a limited and sporadic distribution among eukaryotes. Within metazoans, only nematodes are known to process polycistronic pre-mRNAs, produced from operon units
Jeong Ho Chang et al.
Nature structural & molecular biology, 19(10), 1011-1017 (2012-09-11)
Recent studies showed that Rai1 is a crucial component of the mRNA 5'-end-capping quality-control mechanism in yeast. The yeast genome encodes a weak homolog of Rai1, Ydr370C, but little is known about this protein. Here we report the crystal structures
Anish Das et al.
Nucleic acids research, 45(13), 7886-7896 (2017-06-03)
A unique feature of RNA polymerase II (RNA pol II) is its long C-terminal extension, called the carboxy-terminal domain (CTD). The well-studied eukaryotes possess a tandemly repeated 7-amino-acid sequence, called the canonical CTD, which orchestrates various steps in mRNA synthesis.
Alan R Prescott et al.
Journal of cell science, 127(Pt 4), 812-827 (2013-12-21)
The biogenesis of splicing snRNPs (small nuclear ribonucleoproteins) is a complex process, beginning and ending in the nucleus of the cell but including key stages that take place in the cytoplasm. In particular, the SMN (survival motor neuron) protein complex

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