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MABF147E

Sigma-Aldrich

Anti-Ly-6G (mouse), clone RB6-8C5, biotin conjugate Antibody

clone RB6-8C5, from rat, purified by affinity chromatography

Synonym(s):

Lymphocyte antigen 6G (Ly-6G), Ly-6G.1, Ly-6G

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.45

biological source

rat

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

RB6-8C5, monoclonal

purified by

affinity chromatography

species reactivity

mouse

packaging

antibody small pack of 25 μg

technique(s)

flow cytometry: suitable

UniProt accession no.

P35461

target post-translational modification

unmodified

Gene Information

mouse ... Ly6G(546644)

General description

The RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes.Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports suggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C (Nagendra S. and Schlueter AJ. 2003. Cytometry A, 58(2): 195-200.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This rat monoclonal Anti-Ly-6G (mouse), clone RB6-8C5, biotin conjugate, Cat. No. MABF1479 is validated for use in Flow Cytometry.

Quality

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted biotin removed from the preparation.

Flow Cytometry Analysis: 0.06 μg from a representative lot detected Ly-6G in one million C57Bl/6 bone marrow cells.

Physical form

Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Purified by size-exclusion chromatography

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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