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Key Documents

MAB2056

Sigma-Aldrich

Anti-Integrin α3 Antibody, clone ASC-1

clone ASC-1, Chemicon®, from mouse

Synonym(s):

CD49c

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

ASC-1, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ITGA3(3675)

Specificity

Human alpha3 integrin (CD49c)

Immunogen

Human squamous cell carcinoma cells (SCC9)

Application

Anti-Integrin α3 Antibody, clone ASC-1 detects level of Integrin α3 & has been published & validated for use in FC, IP, IH.
Inhibition of cell attachment: inhibits SCC9 cell adhesion to human and

EHS laminin, and laminin GD-2 peptide. Potentiates inhibition of SKOV-3 cell adhesion to EHS laminin by anti-integrin alpha 5 antibody GoH3. Does not inhibit cell adhesion to fibronectin or collagen type IV.

Immunohistochemistry: on acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue.

Flow cytometry: stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelial cells (HUVEC).

Immunoprecipitation: precipitates PAGE bands of 130kDa (reduced) or 116kDa and 135kDa (non-reduced) from surface-biotinylated SCC9 or SKOV-3 cells.

Working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Integrins

Linkage

Replaces: CBL462B

Physical form

Format: Purified
Liquid in 0.02M phosphate buffer, pH 7.6, 0.25M NaCl containing 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Paul Day et al.
International journal of oncology, 39(4), 863-871 (2011-07-02)
Integrins, a family of heterodimeric receptors for cell adhesion to the extracellular matrix (ECM), play key roles in cell migration, cancer progression and metastasis. As transmembrane proteins, integrins are transported in vesicles and delivered to the cell surface by vesicular
Edith Hintermann et al.
The Journal of cell biology, 153(3), 465-478 (2001-05-02)
Keratinocytes and other epithelial cells express two receptors for the basement membrane (BM) extracellular matrix component laminin-5 (Ln-5), integrins alpha 3 beta 1 and alpha 6 beta 4. While alpha 3 beta 1 mediates adhesion, spreading, and migration (Kreidberg, J.A.
M Shang et al.
The Journal of biological chemistry, 276(35), 33045-33053 (2001-06-08)
Laminins are a family of extracellular matrix glycoproteins involved in cell adhesion and migration. A major obstacle to understanding their structure-function relationships is the lack of small laminin domains capable of replicating integrin-binding, cell-adhesive, and migratory functions of the intact
Julia K L Woodward et al.
Investigative ophthalmology & visual science, 46(10), 3473-3477 (2005-09-28)
TGFbeta has been shown to have a regulatory effect on uveal melanoma invasion, but it is not known which processes are specifically influenced. The purpose of this study was to analyze the effect of TGFbeta stimulation on the adhesive interactions
Expression of laminin isoforms, receptors, and binding proteins unique to nucleus pulposus cells of immature intervertebral disc.
Chen, J; Jing, L; Gilchrist, CL; Richardson, WJ; Fitch, RD; Setton, LA
Connective tissue research null

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