HANG2MAG-12K

MILLIPLEX^® Human Angiogenesis Panel 2, HANG2MAG-12K

Angiogenesis Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple angiogenic biomarkers in human serum, plasma and cell culture samples.

• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

Properties

• Quality Level: 200
• species reactivity: human
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 88-97%; standard curve range: 137-100,000 pg/mL; (Thrombospondin-2); standard curve range: 137-100,000 pg/mL; (sVEGFR2); standard curve range: 137-100,000 pg/mL; (suPAR); standard curve range: 14-10,000 pg/mL; (sAXL); standard curve range: 14-10,000 pg/mL; (sHER3); standard curve range: 14-10,000 pg/mL; (sVEGFR1); standard curve range: 27-20,000 pg/mL; (sIL-6Rα); standard curve range: 27-20,000 pg/mL; (sPECAM-1); standard curve range: 27-20,000 pg/mL; (sTIE-2); standard curve range: 274-200,000 pg/mL; (Angiostatin); standard curve range: 274-200,000 pg/mL; (sE-Selectin); standard curve range: 274-200,000 pg/mL; (sNeuropilin-1); standard curve range: 41-30,000 pg/mL; (Osteopontin (OPN)); standard curve range: 41-30,000 pg/mL; (sEGFR); standard curve range: 41-30,000 pg/mL; (sHER2); standard curve range: 41-30,000 pg/mL; (sc-Kit/sSCFR); standard curve range: 412-300,000 pg/mL; (sVEGFR3); standard curve range: 69-50,000 pg/mL; (sHGFR); standard curve range: 7-5,000 pg/mL; (PDGF-AB/BB)
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

Angiogenesis, the development of new vascular networks, is a key process in normal growth and development as well as in wound healing, with homeostasis maintained by a delicate balance of angiogenic factors and inhibitors. Consequently, insufficient, or excessive blood vessel growth underlies many diseases, including cardiovascular disease, diabetic ulcers, macular degeneration, and cancer. Angiogenesis plays a significant role in tumor growth and metastasis.

MILLIPLEX^® Human Angiogenesis Magnetic Bead Panel 2 is a 19-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, cell culture samples, and tissue homogenates/lysates: Angiostatin, soluble E-Selectin, (sE-Selectin), Osteopontin (OPN), Platelet Derived Growth Factor-AB/BB (PDGF-AB/BB), soluble Platelet Endothelial Cell Adhesion Molecule-1 (sPECAM-1), Thrombospondin-2 (TSP-2), soluble AXL (sAXL), soluble c-Kit/Stem Cell Growth Factor Receptor (sc-Kit/SCFR), soluble Epidermal Growth Factor Receptor (sEGFR)†, soluble Human Epidermal Growth Factor Receptor 2 (sHer2), soluble Human Epidermal Growth Factor Receptor 3 (sHer3), soluble Hepatocyte Growth Factor Receptor/c-Met (sHGFR/c-Met), soluble IL-6Rα, soluble Neuropilin-1 (sNRP-1), soluble Tie-2 (sTie-2), soluble Urokinase-type Plasminogen Activator Receptor (suPAR), soluble Vascular Endothelial Growth Factor Receptor 1 (sVEGFR1), soluble Vascular Endothelial Growth Factor Receptor 2 (sVEGFR2), and soluble Vascular Endothelial Growth Factor Receptor 3 (sVEGFR3). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

†Antibody against active site results in preferential binding to unbound EGFR.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Circulating Cancer

Application

• Analytes: Angiostatin, soluble AXL (sAXL) , soluble c-Kit/Stem Cell Growth Factor Receptor (sc-Kit/SCFR), soluble E-Selectin (sE-Selectin), soluble Epidermal Growth Factor Receptor (sEGFR/sErbB1/sHER1)†, soluble Human Epidermal Growth Factor Receptor 2 (sHer2/sHER2/sErbB2), soluble Human Epidermal Growth Factor Receptor 3 (sHer3/ sHER3 / sErbB3), soluble Hepatocyte Growth Factor Receptor (sHGFR/c-Met/sc-Met), soluble IL-6Rα (IL-6Rα (soluble)/sIL-6Rα), soluble Neuropilin-1 (sNRP-1), Osteopontin (OPN), Platelet Derived Growth Factor-AB/BB (PDGF-AB/BB), soluble Platelet Endothelial Cell Adhesion Molecule-1 (sPECAM-1/sCD31), Thrombospondin-2 (TSP-2), soluble Tie-2 (sTie-2), soluble Urokinase-type Plasminogen Activator Receptor (suPAR), soluble Vascular Endothelial Growth Factor Receptor 1 (soluble VEGFR1, sVEGFR1/ sFlt-1), soluble Vascular Endothelial Growth Factor Receptor 2 (soluble VEGFR2/sVEGFR2/ sKDR/sFlk-1), soluble Vascular Endothelial Growth Factor Receptor 3 (soluble VEGFR3/sVEGFR3/ sFlt-4).
• †Antibody against active site results in preferential binding to unbound EGFR.
• Recommended Sample Type: Human serum, plasma, cell culture supernatants and tissue/cell homogenates/lysates
• Recommended Sample Dilution: 25 μL per well of 1:5 diluted plasma or serum; cell culture supernatant may be used undiluted or diluted with appropriate control medium
• Assay Run Time: Overnight (16-18 hours) at 2-8°C
• Research Category: Cancer

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Please contact Technical Service for linearity of dilution.

Sensitivity: See kit protocol for individual analytes sensitivities.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Safety Information

• Pictograms: GHS06,GHS08,GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H302 + H332,H311,H317,H318,H373,H411
• Precautionary Statements: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• Target Organs: Respiratory Tract
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects