AB2237
Anti-PAX6 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Aniridia type II protein, paired box 6, paired box gene 6, paired box gene 6 (aniridia, keratitis), paired box homeotic gene-6
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat, chicken
should not react with
human
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PAX6(5080)
General description
PAX6 is the most researched of the PAX genes and appears throughout the literature as a "master control" gene for the development of eyes and other sensory organs, certain neural and epidermal tissues as well as other homologous structures, usually derived from ectodermal tissues. This transcription factor is most famous for its use in the interspecifically induced expression of ectopic eyes and is of medical importance because heterozygous mutants produce a wide spectrum of ocular defects such as Aniridia in humans. This gene encodes paired box gene 6, one of many human homologues of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause aniridia as well as Peter′s anomaly, both ocular diseases.
Specificity
This antibody recognizes the C-Terminus of PAX6.
Immunogen
KLH conjugated followed by PAX6 corresponding to the C-Terminus
Application
Anti-PAX6 Antibody detects level of PAX6 & has been published & validated for use in WB & IC.
Immunocytochemistry:
Working dilutions of 1:50 - 1:1,000 of Anti-Pax6 was used to resolve the protein in chicken spinal cord.
Working dilutions of 1:50 - 1:1,000 of Anti-Pax6 was used to resolve the protein in chicken spinal cord.
Quality
Routinely evaluated by Western Blot on rat and mouse brain lysates.
Western Blot Analysis:
0.5 µg/mL of this lot detected PAX6 on 10 µg of mouse and rat brain lysates.
Western Blot Analysis:
0.5 µg/mL of this lot detected PAX6 on 10 µg of mouse and rat brain lysates.
Target description
~48 kDa
Linkage
Replaces: AB5409
Physical form
Immunoaffinity purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.
Analysis Note
Control
Mouse and rat brain lysates
Mouse and rat brain lysates
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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