560000
Sputolysin® Reagent
Intended for the isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum.
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About This Item
Quality Level
form
liquid
reaction suitability
reagent type: reductant
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
protect from light
shipped in
ambient
storage temp.
15-25°C
InChI
1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m0/s1
InChI key
VHJLVAABSRFDPM-IMJSIDKUSA-N
Related Categories
General description
A concentrate of dithiothreitol (DTT) in phosphate buffer. Useful for the isolation of pathogenic or saprophytic bacteria, fungi, yeast, and epithelial cells from sputum and other mucoid fluids.
Concentrate of Dithiothreitol (DTT; Cat. No. 233155) in phosphate buffer, pH 6.5-7.5. Intended for the isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum. Each vial is sufficient to make 100 ml of working solution.
Note: 1 set = 20 x 10 ml.
Warning
Toxicity: Harmful (C)
Physical form
20 vials each containing 10 ml of concentrated DTT OR 1 vial containing 10 ml of concentrated DTT.
Preparation Note
Dilute the contents of one vial to 100 ml with sterile distilled water. Swirl gently to dissolve any crystals. If the diluted material appears cloudy, the solution may be filtered to remove any insoluble particles. The diluted solution is ~6.5 mM dithiothreitol in 100 mM phosphate buffer, pH 7.0.
Reconstitution
Once a vial is opened the unused concentrate should be purged with an inert gas (e.g. N₂) and refrigerated (4°C). Diluted solutions, stored aseptically under an inert gas, are stable for up to 48 h at 4°C.
Other Notes
Hirsch, S.R., et al. 1969. J. Lab. Clin. Med.74, 346.
Shah, R.R. and Dye, W.E. 1966. Am. Rev. Respir. Dis.94, 454.
Dixon, J.M.S. and Miller, D.C. 1965 (November). Lancet p. 1046.
Cleland, W.W. 1964. Biochemistry3, 480.
May, J.R. 1953 (September). Lancet p. 535.
Shah, R.R. and Dye, W.E. 1966. Am. Rev. Respir. Dis.94, 454.
Dixon, J.M.S. and Miller, D.C. 1965 (November). Lancet p. 1046.
Cleland, W.W. 1964. Biochemistry3, 480.
May, J.R. 1953 (September). Lancet p. 535.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
SPUTOLYSIN is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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