1.08339

Sabouraud Dextrose broth

GranuCult^® prime, Dextrose 2 %, according to EP, USP, JP, granular, for yeasts, for molds, pkg of 500 g

• Synonym(s): SDB, Sabouraud Dextrose Bouillon
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• Agency: JP; USP; according to EP
• Quality Level: 200
• sterility: non-sterile
• form: granular
• composition: Dextrose, 2%
• packaging: pkg of 500 g
• manufacturer/tradename: GranuCult^® prime
• storage condition: dry media (Keep tightly closed)
• technique(s): microbiological culture: suitable
• color: light yellow
• pH: 5.5-5.7 (22 °C, 30 g/L in H₂O, after autoclaving)
• solubility: 30 g/L
• bulk density: 640 kg/m³
• application(s): clinical; microbiology
• storage temp.: 15-25°C
• suitability: molds; yeasts

Description

General description

GranuCult^® prime SABOURAUD-2% dextrose broth (According to harm. EP/USP/JP) is used for the cultivation and isolation of yeasts and molds as well as the absence test for Candida albicans. The high concentration of dextrose, in addition to the low pH, promotes the growth, the formation of spores (Conidia and Sporangia), and the formation of pigments of yeasts and molds. It also inhibits the growth of bacteria.

Application

GranuCult^® prime SABOURAUD-2% dextrose broth is suitable for the cultivation of dermatophytes, yeasts, and molds. It can also be used for sterility testing and the determination of the fungi-static activity of pharmaceutical and cosmetical products.

Features and Benefits

• GranuCult^® offers superior granulated culture media
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
• Excellent wettability, solubility, and free-flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands
• Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): clear
Appearance (colour): yellowish-brown
pH-value (25 °C): 5.4 - 5.8
Typical composition (g/litre): Peptone from meat 5.0; Peptone from casein 5.0; D(+)Glucose 20.0.
Growth (Trichophyton mentagrophytes ATCC 18748): good to very good
Growth (Trichophyton rubrum ATCC 28188): fair to good
Growth (Microsporum gallinae ATCC 12108): fair to good
Growth (Trichophyton ajelloi ATCC 28454): fair to good
Growth (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): good to very good
Growth (Penicillium commune ATCC 10428): good to very good
Incubation: 7 days; 28°C
Growth promotion test in accordance with the harmonised method of EP, USP and JP.
Inoculum on reference medium (Candida albicans ATCC 10231 (WDCM 00054)): ≤ 100
Growth (Candida albicans ATCC 10231 (WDCM 00054)): fair to very good
Incubation: up to 3 days; 30-35°C
The information above is current at this time of publication and is subject to change without notice (except for customers holding a change control agreement with our company). The information/format can be altered at any time and inany way if internal company matters or Standard Regulationsdo say so.

Other Notes

• The appearance of the broth is clear and yellowish-brown.
• The pH value at 25 °C is in the range of 5.4-5.8.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3