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Key Documents

1.05175

Sigma-Aldrich

Hematoxylin solution modified acc. to Gill II

for microscopy

Synonym(s):

Hematoxylin solution acc. to GILL

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About This Item

UNSPSC Code:
41116113
NACRES:
NA.41

Quality Level

form

liquid

IVD

for in vitro diagnostic use

technique(s)

microbe id | staining: suitable

pH

2.5 (25 °C in H2O)

density

1.04-1.06 g/cm3 at 20 °C

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

storage temp.

15-25°C

General description

Hematoxylin solution modified acc. to Gill II - for microscopy, is used for human-medical cell diagnosis and serves the purpose of the cytological and histological investigation of sample material of human origin. It is a ready-to-use staining solution that when used together with other in vitro diagnostic products from our portfolio makes cytological target structures evaluable for diagnostic purposes in human gynecological and clinico-cytological specimen materials, for example cervical smears. In hematoxylin-eosin staining (H&E), the most widely used histological staining technique, nuclear staining is achieved using ready-to-use staining solutions, such as Mayer′s hemalum solution or Hematoxylin solution modified according to Gill II or Gill III. The dye is oxidized in a controlled manner, which renders the results robust and allows a brilliant differentiation of the stained structures. Counterstaining of i.e. for proteins, collagen, keratin, intercellular substances can be performed with alcoholic or aqueous Eosin Y solution 0.5 %.These solutions do need to be filtrated prior use.
A 500 ml bottle is sufficient for 1500 - 2500 slides. It is registered as IVD and certified as CE product. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Suitability for microscopy: passes test
Nuclei (Vaginal smear): blue to dark violet
Cyanophilic cytoplasm: bluish green to green
Eosinophilic cytoplasm: pink

Pictograms

Health hazardExclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - STOT RE 2 Oral

Target Organs

Kidney

Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

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