V900892
Collagenase from Clostridium histolyticum
Type II, Vetec™, reagent grade, powder, ≥125 CDU/mg solid
Synonim(y):
Clostridiopeptidase A
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Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
EC Number:
232-582-9
MDL number:
Specific activity:
≥125 CDU/mg solid
Pomoc techniczna
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Pozwól nam pomócgrade
reagent grade
product line
Vetec™
form
powder
specific activity
≥125 CDU/mg solid
storage temp.
−20°C
Application
Collagenase from Clostridium histolyticum may be used for the isolation of cells from many types of animal tissue. VETEC Collagenase Type II has higher clostripain activity, and is suggested for bone, heart, liver, thyroid and salivary primary cell isolations.
Biochem/physiol Actions
Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.
Analysis Note
Also contains clostripain, nonspecific neutral protease, and tryptic activities.
Other Notes
One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.
Legal Information
Vetec is a trademark of Merck KGaA, Darmstadt, Germany
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Klasa składowania
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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