Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.
Wybierz wielkość
Zmień widok
Informacje o tej pozycji
UNSPSC Code:
41115711
NACRES:
NB.21
L × i.d.:
7.5 cm × 8 mm
Particle size:
10 μm
Matrix active group:
ether phase
Matrix:
hydroxylated methacrylic polymer
Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomócNazwa produktu
TSKgel® Ether-5PW HPLC Column, phase ether, L × I.D. 7.5 cm × 8 mm, 10 μm particle size
material
glass
product line
TSKgel®
mol wt
2000 - 1,000,000
manufacturer/tradename
Tosoh 14014
parameter
≤4 M salt concentration, ≤50% organic solvent, 1.2 mL/min flow rate, 10-50 °C temperature, 20 bar max. pressure
technique(s)
HPLC: suitable
L × I.D.
7.5 cm × 8 mm
matrix
hydroxylated methacrylic polymer
matrix active group
ether phase
particle size
10 μm
operating pH
2-12
separation technique
hydrophobic interaction (HIC)
General description
Both hydrophobic interaction chromatography (HIC) and reversed-phase liquid chromatography separate on the basis of protein hydrophobicity and allow selective binding and desorption of proteins. However, HIC operates at significantly lower binding energy and uses aqueous mobile phases. These characteristics provide a gentle technique that is less likely to disturb protein conformation. As a result, HIC generally provides better activity recovery.
Three resin-based TSKgel HIC columns provide a range of hydrophobicities for chromatographic optimization. TSKgel Phenyl-5PW columns are most hydrophobic, followed by Ether-5PW, while the surface of Butyl-NPR columns is most polar or least hydrophobic. Ammonium sulfate concentration can be minimized by using a more hydrophobic packing such as Phenyl-5PW. We recommend the more polar Ether-5PW column for purifying more hydrophobic proteins. Ether-5PW and Phenyl-5PW packings are based on TSKgel G5000PW resin - 10 micron particles with 1000  pores. Butyl-NPR packing is prepared from 2.5 micron nonporous particles, allowing for rapid analyses and semi-micropreparative applications. All three column types can be cleaned with 0.2 M NaOH. Scale-up can be performed by using corresponding Toyopearl bulk resins.
Three resin-based TSKgel HIC columns provide a range of hydrophobicities for chromatographic optimization. TSKgel Phenyl-5PW columns are most hydrophobic, followed by Ether-5PW, while the surface of Butyl-NPR columns is most polar or least hydrophobic. Ammonium sulfate concentration can be minimized by using a more hydrophobic packing such as Phenyl-5PW. We recommend the more polar Ether-5PW column for purifying more hydrophobic proteins. Ether-5PW and Phenyl-5PW packings are based on TSKgel G5000PW resin - 10 micron particles with 1000  pores. Butyl-NPR packing is prepared from 2.5 micron nonporous particles, allowing for rapid analyses and semi-micropreparative applications. All three column types can be cleaned with 0.2 M NaOH. Scale-up can be performed by using corresponding Toyopearl bulk resins.
Legal Information
TSKgel is a registered trademark of Tosoh Corporation
Ta strona może zawierać tekst przetłumaczony maszynowo.
Still not finding the right product?
Explore all of our products under TSKgel® Ether-5PW HPLC Column
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.