To regenerate this column after experiencing changes in selectivity for sugar analysis, follow these flushing steps:
1. Flush with warm (60 °C) distilled water
2. 50 mL methanol
3. 50 mL acetonitrile
4. 25 mL methanol
5. 25 mL mobile phase
Evaluate column performance.
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58338
SUPELCOSIL™ LC-NH₂ (5 µm) HPLC Columns
L × I.D. 25 cm × 4.6 mm, HPLC Column
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| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
1 ea | Dostępne do wysyłki DZISIAJzSchnelldorf Distribution | 4260,00 zł |
Informacje o tej pozycji
UNSPSC Code:
41115700
eCl@ss:
32110501
EC Number:
276-161-8
NACRES:
NB.21
L × i.d.:
25 cm × 4.6 mm
Particle size:
5 μm
Matrix active group:
NH2, amino phase
Pore size:
120 Å
Matrix:
silica gel, spherical particle platform
4260,00 zł
Dostępne do wysyłki DZISIAJSzczegóły
Pomoc techniczna
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Pozwól nam pomócNazwa produktu
SUPELCOSIL™ LC-NH2 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
agency
suitable for USP L8
Quality Segment
feature
endcapped
manufacturer/tradename
SUPELCOSIL™
extent of labeling
3% Carbon loading
parameter
≤70 °C temp. range, 400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
L × I.D.
25 cm × 4.6 mm
surface area
170 m2/g
surface coverage
surface coverage 5.1 μmol/m2
matrix
silica gel, spherical particle platform
matrix active group
NH2, amino phase
particle size
5 μm
pore size
120 Å
application(s)
food and beverages
separation technique
hydrophilic interaction (HILIC), normal phase
General description
Silica-based SUPELCOSIL LC-NH2 columns separate monosaccharides, disaccharides, and some trisaccharides, with elution generally in order of increasing molecular weight. Retention decreases as the proportion of water:acetonitrile in the mobile phase is increased. For more information, request T397126 (Sugars on SUPELCOSIL LC-NH2 Columns).
The amino column is most often employed for the separation of mono- and disaccharides. As a normal-phase application, amino columns are used in the petroleum industry (see SUPELCOSIL™ LC-NH2-NP HPLC Columns for additional details).
Application
SUPELCOSIL™ LC-NH2 (5 µm) HPLC Columns can be effectively utilized for the separation of mono- and disaccharides and other separations of Sugar and Carbohydrates.
Under normal-phase conditions, amino columns are also used in the petroleum industry.
Under normal-phase conditions, amino columns are also used in the petroleum industry.
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
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Ta pozycja | |||
|---|---|---|---|
| description 5 μm particle size, L × I.D. 25 cm × 4.6 mm | description 3 μm particle size, L × I.D. 7.5 cm × 4.6 mm | description 3 μm particle size, L × I.D. 15 cm × 4.6 mm | description 3 μm particle size, L × I.D. 15 cm × 3 mm |
| matrix active group NH2, amino phase | matrix active group NH2, amino phase | matrix active group NH2, amino phase | matrix active group NH2, amino phase |
| separation technique normal phase, hydrophilic interaction (HILIC) | separation technique hydrophilic interaction (HILIC), normal phase | separation technique hydrophilic interaction (HILIC), normal phase | separation technique hydrophilic interaction (HILIC), normal phase, reversed phase |
| L × I.D. 25 cm × 4.6 mm | L × I.D. 7.5 cm × 4.6 mm | L × I.D. 15 cm × 4.6 mm | L × I.D. 15 cm × 3 mm |
| particle size 5 μm | particle size 3 μm | particle size 3 μm | particle size 3 μm |
| pore size 120 Å | pore size 120 Å | pore size 120 Å | pore size 120 Å |
| technique(s) HPLC: suitable | technique(s) HPLC: suitable | technique(s) HPLC: suitable | technique(s) HPLC: suitable |
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I have a problem with the column selectivity (SUPELCOSIL LC-NH2) after 500 injections (sugars analysis, 20:80 ACN:H2O, inj 20 uL). The change was gradual. How can I regenerate column? Flush with: warm water, methanol, acetonitrile, methanol, mobile phase?
1 answer-
Helpful?
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
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Is the 25:75 water:acetonitrile used in the test for 58338 SUPELCOSIL™ LC-NH2 measured by volume or by weight?
1 answer-
Volume to volume measurement is used when making the mobile phases.
Helpful?
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