ZRB1116

Anti-CD56 Antibody, clone 3F5 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonim(y): Neural cell adhesion molecule 1;N-CAM-1;NCAM-1
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: 3F5, recombinant monoclonal
• Application: FACS, IHC (p), WB, affinity binding assay
• Citations: -

Właściwości

• biological source: rabbit
• Quality Segment: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 3F5, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 94.57 kDa, observed mol wt ~120 kDa
• purified by: using Protein A
• species reactivity: human, rat
• species reactivity (predicted by homology): bovine, monkey, mouse, feline, canine
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Sustainability features
• technique(s): affinity binding assay: suitable, flow cytometry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal
• Protein ID accession no.: NP_851996.2?
• UniProt accession no.: P13591
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Opis

General description

This product aligns with one or more of the 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Neural cell adhesion molecule 1 (UniProt: P13591; also known as N-CAM-1, NCAM-1, CD56) is encoded by the NCAM1 (also known as NCAM) gene (Gene ID: 4684) in human. CD56 is a member of the immunoglobulin superfamily that is engaged in both so-called homophilic and heterophilic interactions. It is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 20-718), a transmembrane domain (aa 719-739), and a cytoplasmic domain (aa 740-858). It also contains five Ig-like C2-type domains and two fibronectin type-III domains. CD56 is expressed by neurons, glia, skeletal muscle, and natural killer (NK) cells. It is involved in neuron-neuron adhesion, neurite fasciculation, and outgrowth of neurite. It is considered as the archetypal phenotypic marker for natural killer cells, but is also expressed in many other immune cells, including ab T cells, g T cells, dendritic cells, and monocytes. It is also shown to play a role in the fungus mediated NK cell activation and blocking of CD56 surface receptor reduced fungal mediated NK cell activation and reduced cytokine secretion. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Ziegler, S., et al. (2017). Sci. Rep. 7; Article 6138; Van Acker, HH., et al. (2017). Front. Immunol. 8; Article 892).

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Immunogen

KLH-conjugated linear peptide corresponding to 23 amino acids from the N-terminal, extracellular domain of human Neural cell adhesion molecule 1 (CD56).

Application

Quality Control Testing

Evaluated by Western Blotting in Rat brain tissue lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected CD56 in Rat brain tissue lysate.

Tested applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected CD56 in human brain tissue lysate.

Affinity Binding Assay: A representative lot of this antibody bound CD56 with a KD of 1.5 x 10-9 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD56 (NCAM1) in human cerebral cortex tissue sections.

Flow Cytometry Analysis: 1 µg of a representative lot of this antibody detected CD56 in one million human PBMCs.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Biochem/physiol Actions

Clone 3F5 is a Rabbit recombinant monoclonal antibody that specifically detects Neural cell adhesion molecule 1 (CD56). It targets an epitope within 23 amino acids from the N-terminal, extracellular domain.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Preparation Note

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 13 - Non Combustible Solids
• Co się dzieje?: WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable