Y4770
Anti-YAP1 (C-terminal) antibody produced in rabbit
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonim(y):
Anti-YAP, Anti-YAP2, Anti-YAP65, Anti-Yes-associated protein 1, 65kDa
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About This Item
Polecane produkty
pochodzenie biologiczne
rabbit
białko sprzężone
unconjugated
forma przeciwciała
affinity isolated antibody
rodzaj przeciwciała
primary antibodies
klon
polyclonal
Postać
buffered aqueous solution
masa cząsteczkowa
antigen ~65 kDa
reaktywność gatunkowa
human
rozszerzona walidacja
recombinant expression
Learn more about Antibody Enhanced Validation
stężenie
~1 mg/mL
metody
western blot: 0.5-1 μg/mL using HEK-293T cells co-transfected with human YAP1 and c-Abl
numer dostępu UniProt
Warunki transportu
dry ice
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... YAP1(10413)
mouse ... Yap1(22601)
rat ... Yap1(363014)
Opis ogólny
YAP1 (Yes-associated protein 1, YAP, YAP2, YAP65) is a modular adaptor protein with multiple protein interaction domains, that is based on its interaction with Src-family tyrosine kinase c-Yes. It has a SH3-binding motif, proline-rich amino terminus, a WW domain, a coiled-coil and a PDZ-binding motif at the extreme C-terminus. YAP1 also contains a 14-3-3 interacting motif.
Specyficzność
Anti-YAP1 (C-terminal) antibody is specific for human YAP1. The product is expected to react with mouse, bovine, and dog YAP1. In immunoblotting, detection of the YAP1 band is specifically inhibited with the immunizing peptide.
Immunogen
synthetic peptide corresponding to amino acids 442-454 located at the C-terminus of human YAP1. The sequence is identical in mouse, bovine, and dog YAP1.
Zastosowanie
Anti-YAP1 (C-terminal) antibody is suitable for use in western blot (.5-1 μg/mL) using HEK-293T cells co-transfected with human YAP1 and c-Abl.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Western Blotting (1 paper)
Immunoprecipitation (1 paper)
Western Blotting (1 paper)
Działania biochem./fizjol.
YAP1 (Yes-associated protein 1) induces p73-dependent apoptosis through the specific and selective co-activation of p53AIP1, an apoptotic p73 target gene. It stabilizes p73 by preventing Itch-mediated ubiquitination of p73. Upon phosphorylation by Akt at Ser127, 14-3-3 is recruited and stimulates YAP1 localization to the cytoplasm, resulting in loss of co-activator function in the nucleus. Inhibition of Akt potentiates the nuclear re-localization of YAP1 to promote apoptosis by p73.
YAP1 is a WW domain containing protein that functions as a transcriptional co-activator . It may also regulate p73 gene function in response to DNA damage. YAP1 is known to associate with a wide range of transcription factors . Alterations in YAP1 have been associated with tumor development .
Postać fizyczna
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Cennik
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certyfikaty analizy (CoA)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
The transcriptional coactivator Yes-associated protein drives p73 gene-target specificity in response to DNA Damage
Strano S, et al.
Molecular Cell, 18(4), 447-459 (2005)
Akt phosphorylates the Yes-associated protein, YAP, to induce interaction with 14-3-3 and attenuation of p73-mediated apoptosis
Basu S, et al.
Molecular Cell, 11(1), 11-23 (2003)
The Yes-associated protein 1 stabilizes p73 by preventing Itch-mediated ubiquitination of p73
Levy D, et al.
Cell Death and Differentiation, 14(4), 743-743 (2007)
Akihiko Komuro et al.
The Journal of biological chemistry, 278(35), 33334-33341 (2003-06-17)
The ErbB-4 receptor protein-tyrosine kinase is proteolytically processed by membrane proteases in response to the ligand or 12-O-tetradecanoylphorbol-13-acetate stimulation resulting in the cytoplasmic fragment translocating to the cell nucleus. The WW domain-containing co-transcriptional activator Yes-associated protein (YAP) associates physically with
Fernando D Camargo et al.
Current biology : CB, 17(23), 2054-2060 (2007-11-06)
The mechanisms that regulate mammalian organ size are poorly understood. It is unclear whether the pathways that control organ size also impinge on stem/progenitor cells. A highly expressed gene in stem cells is YAP1, the ortholog of Drosophila Yorkie, a
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