T9008

Trypsin inhibitor from Glycine max (soybean)

1% solution in water (contains no buffers)

• Synonim(y): SBTI
• Numer CAS: 9035-81-8
• UNSPSC Code: 12352202
• MDL number: MFCD00082103

Właściwości

• biological source: Glycine max (soybean)
• sterility: sterile-filtered
• form: solution
• mol wt: 20,100 Da
• technique(s): inhibition assay: suitable
• solubility: balanced salt solution: 1 mg/mL, concentrate: >10 mg/mL, hazy, amber-yellow, phosphate buffer: 10 mg/mL, water: 10 mg/mL, serum-free medium: soluble
• shipped in: ambient
• storage temp.: 2-8°C

Opis

Biochem/physiol Actions

This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.

Preparation Note

Prepared From Type I-S

The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet.

Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.

Analysis Note

Protein determined by biuret. One mg of trypsin inhibitor will inhibit 1.0-2.5 mg of trypsin of ~10000 BAEE units/mg trypsin.

Other Notes

One trypsin unit = A₂₅₃ of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

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Informacja o środkach bezpieczeństwa

• pictograms: GHS08
• signalword: Danger
• hcodes: H317,H334
• pcodes: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Klasa składowania: 12 - Non Combustible Liquids
• wgk: WGK 2
• ppe: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter