T7818

Trizma^® Pre-set crystals

BioPerformance Certified, pH 7.5, average M_w 150.6

• Synonim(y): Tris crystals
• Kod UNSPSC: 12161700
• NACRES: NA.25

Właściwości

• klasa czystości: BioPerformance Certified
• Postać: crystalline powder
• masa cząsteczkowa: average M_w 150.6
• metody: cell culture | mammalian: suitable
• zanieczyszczenia: DNase, RNase, Protease, NICKase, none detected; endotoxin, tested; total aerobic microbial count, tested; ≤0.05 A₂₉₀; ≤0.5% water (Karl Fischer); ≤5 ppm Heavy metals (as Pb)
• pH: 7.50 (25 °C, 0.1 M)(lit.); 7.5
• przydatny zakres pH: 7.0-9.0
• rozpuszczalność: water: 0.66 g/mL, clear, colorless
• Zastosowanie: diagnostic assay manufacturing

Opis

Zastosowanie

Dissolve in water to make a 0.1M solution. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.

Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.

If a different concentration is desired, note that there is a concentration effect on the pH of the solution.

The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.

Inne uwagi

This product is a pre-mixed combination of Trizma base and Trizma HCl that can be used to prepare a common pH value for Tris buffer.

Certain pH electrodes do not give accurate results with Tris buffers.

Uwaga dotycząca przygotowania

Trizma solutions can be autoclaved.

Informacje prawne

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Środki ochrony indywidualnej: dust mask type N95 (US), Eyeshields, Gloves