T2885

3,3′,5,5′-Tetramethylbenzidine

TMB substrate, chromogenic, ≥98% (TLC), powder

• Synonim(y): BM blue, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate
• Wzór liniowy: [-C₆H₂(CH₃)₂-4-NH₂]₂
• Numer CAS: 54827-17-7
• Masa cząsteczkowa: 240.34
• Beilstein: 2808541
• Numer WE: 259-364-6
• Numer MDL: MFCD00007748
• Kod UNSPSC: 12352204
• Identyfikator substancji w PubChem: 24900090
• NACRES: NA.83

Właściwości

• product name: 3,3′,5,5′-Tetramethylbenzidine, ≥98% (TLC)
• Próba: ≥98% (TLC)
• Postać: powder
• mp: 168-171 °C (lit.)
• rozpuszczalność: ethyl acetate: 50 mg/mL, clear to very slightly hazy
• temp. przechowywania: 2-8°C
• ciąg SMILES: Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
• InChI: 1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
• Klucz InChI: UAIUNKRWKOVEES-UHFFFAOYSA-N

Opis

Zastosowanie

3,3′,5,5′-Tetramethylbenzidine has been used:

• as a supplement in Man-Rodosa-Sharpe (MRS) agar plate, to stains the lactobacilli colonies blue in the presence of O2
• as a substrate solution for ELISA (enzyme-linked immunosorbent assay)
• in myeloperoxidase activity assay to quantify neutrophil

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

Działania biochem./fizjol.

3,3′,5,5′-Tetramethylbenzidine/TMB can be used as a chromogen to increase the progression of product obtained in peroxidase reaction. In food and environmental decontamination procedures, TMB can be used in in situ free available chlorine (FAC) monitoring of chlorite-based sanitizers.

Opakowanie

Bottomless glass bottle. Contents are inside inserted fused cone.

Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08,GHS07
• Hasło ostrzegawcze: Warning
• Zwroty wskazujące rodzaj zagrożenia: H302,H351,H413
• Zwroty wskazujące środki ostrożności: P202 - P264 - P273 - P280 - P301 + P312 - P308 + P313
• Klasyfikacja zagrożeń: Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: dust mask type N95 (US), Eyeshields, Faceshields, Gloves