T0256

Trypsin inhibitor from bovine pancreas

Type I-P, essentially salt-free, lyophilized powder

• Synonim(y): BPTI
• Numer CAS: 9087-70-1
• UNSPSC Code: 12352202
• EC Number: 232-906-9
• MDL number: MFCD00082102

Właściwości

• biological source: bovine pancreas
• type: Type I-P
• form: essentially salt-free, lyophilized powder
• mol wt: ~6500 Da (single-chain 58-amino acid peptide)
• purified by: crystallization
• technique(s): inhibition assay: suitable
• solubility: water: 10 g/L
• storage temp.: −20°C

Opis

Biochem/physiol Actions

A 58-residue protein whose binding to various serine proteases has been extensively studied. It is highly homologous to a family of mamba snake dendrotoxin proteins that inhibit various K⁺ channels. It also binds to an intracellular site associated with the large conductance Ca²⁺-activated K⁺ channel..

Preparation Note

Prepared by method of Kunitz and Northrup, J. Gen. Physiol., 19, 991 (1936).

Analysis Note

One mg of trypsin inhibitor will inhibit greater than 1.5 mg of trypsin with activity of approximately 10,000 BAEE units per mg protein.

Protein determined by biuret.

Other Notes

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.

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Informacja o środkach bezpieczeństwa

• pictograms: GHS08
• signalword: Danger
• hcodes: H317,H334
• pcodes: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• ppe: Eyeshields, Gloves, type N95 (US)