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SRP6112

G6PD from E. coli

Name: G6PD from <I>E. coli</I>
Brand: SIGMA

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonim(y):

Glucose-6-phosphate 1-dehydrogenase, Zwf

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Informacje o tej pozycji

Numer CAS:

9001-40-5

NACRES:

NA.32

UNSPSC Code:

12352204

eCl@ss:

32035490

Form:

liquid

Assay:

≥90% (SDS-PAGE)

Biological source:

Escherichia coli

Recombinant:

expressed in E. coli

Mol wt:

55.7 kDa (491 aa, 1-491 aa)

Pomoc techniczna

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Pozwól nam pomóc

Właściwości

biological source

Escherichia coli

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

liquid

mol wt

55.7 kDa (491 aa, 1-491 aa)

packaging

pkg of 100 μg

NCBI accession no.

P0AC53

UniProt accession no.

P0AC53

shipped in

dry ice

storage temp.

−70°C

Gene Information

Escherichia coli ... ZWF(7149716)

Opis

General description

Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH.

Specific activity is 8-10 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP.

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase (G6PD) converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produces NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency causes acute hemolytic anemia.

Physical form

1 mg/mL solution in 50 mM MES pH 6.0, 0.1 mM PMSF, 2 mM EDTA, 0.5 mM DTT and 10% glycerol.

Preparation Note

Centrifuge the vial prior to opening.

Other Notes

MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E

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Klasa składowania

11 - Combustible Solids

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)