SRP5162

4EBP1, His tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonim(y): BP-1, EIF4EBP1, MGC4316, PHAS-I
• Numer CAS: 185703-54-2
• NACRES: NA.32
• UNSPSC Code: 12352202
• Assay: ≥70% (SDS-PAGE)
• Biological source: human
• Recombinant: expressed in baculovirus infected Sf9 cells

Właściwości

• biological source: human
• recombinant: expressed in baculovirus infected Sf9 cells
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• mol wt: ~18 kDa
• NCBI accession no.: NM_004095
• application(s): cell analysis
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... EIF4EBP1(1978)

Opis

General description

Eukaryotic translation initiation factor 4E binding protein 1 (E1F4EBP1) is part of the translation repressor protein family. The gene encoding it is localized on human chromosome 8p11-p12.

Biochem/physiol Actions

Eukaryotic translation initiation factor 4E binding protein 1 (E1F4EBP1) is involved in protein synthesis, cell growth and survival. In the unphosphorylated form, it suppresses the translation initiation of capped mRNAs, whereas in phosphorylated form, it increases translation initiation. Phosphorylation of E1F4EBP1 releases it from eukaryotic translation initiation factor 4E (eIF4E) and this results in cap-dependent translation. The protein is also involved in tumorigenesis and the gene is overexpressed in various carcinomas. It is also a substrate for mTORC1 (mammalian target of rapamycin complex 1). In non-phosphorylated conditions, E1F4EBP1 interacts with p21 (cyclin-dependent kinase inhibitor) and enhances destabilization of p21.

Physical form

Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

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Informacja o środkach bezpieczeństwa

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H360D
• pcodes: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Klasa składowania: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 1