SRP5160

14-3-3 σ, untagged human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonim(y): 14-3-3 σ, SFN, YWHAS, stratifin
• Numer CAS: 1173938-21-0
• NACRES: NA.32
• UNSPSC Code: 12352202
• Form: buffered aqueous glycerol solution
• Assay: ≥70% (SDS-PAGE)
• Biological source: human
• Recombinant: expressed in E. coli
• Mol wt: ~29 kDa

Właściwości

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• mol wt: ~29 kDa
• NCBI accession no.: NM_006142
• application(s): cell analysis
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... SFN(2810)

Opis

General description

14-3-3σ or stratifin is a protein that is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3σ is mediated by a p53 responsive element. Exogenous introduction of 14-3-3σ into cycling cells results in a G2 cell cycle arrest. Knockout of 14-3-3σ in cells showed that the cells are unable to maintain cell cycle arrest after DNA damage. The 14-3-3σ -/- cells die as they enter mitosis. This process is associated with a failure of the 14-3-3σ-deficient cells to sequester the proteins that initiate mitosis and prevent them from entering the nucleus. Thus, 14-3-3σ plays an important role in maintaining the G2 checkpoint in cells and preventing mitotic death.

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 10 - Combustible liquids
• Co się dzieje?: WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable