SRP5127

RhoA, His tagged human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonim(y): ARH12, ARHA, RHO12, RHOH12
• NACRES: NA.32
• UNSPSC Code: 12352200
• eCl@ss: 32160703
• Form: buffered aqueous glycerol solution
• Assay: ≥70% (SDS-PAGE)
• Biological source: human
• Recombinant: expressed in E. coli
• Mol wt: ~23 kDa

Właściwości

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• mol wt: ~23 kDa
• NCBI accession no.: NM_001664
• application(s): cell analysis
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... RHOA(387)

Opis

General description

Ras homolog family member A (RhoA) is a small GTPase which is part of the Rho gene family. The gene encoding it is localized on human chromosome 3p21.31.

Application

RhoA, His tagged human has been used for in vitro kinase assay.

Biochem/physiol Actions

Ras homolog family member A (RhoA) interacts with protein kinases and serves as a target of activated GTPase. RhoA plays an important role in the initial events of cell protrusion. Rho mediates corneal epithelial migration in response to external stimuli by regulating the organization of the actin cytoskeleton. The inhibition of RhoA signaling is required for both vaccinia morphogenesis and virus-induced cell motility. Vaccinia inhibits RhoA signaling using the viral protein F11 and this can enhance the spread of infection. The protein has a role in basal body docking and planar polarization.

Physical form

Supplied in 50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08,GHS07
• Słowo ostrzegawcze: Danger
• Kody zagrożeń: H315,H319,H360D,EUH451
• Ostrzeżenia: P201 - P202 - P273 - P308 + P313 - P391 - P501
• Hazard Classifications: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2 - vPvM
• Klasa składowania: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable